Neuroprotective compounds of Tilia amurensis.

作者信息

Lee Bohyung, Weon Jin Bae, Eom Min Rye, Jung Youn Sik, Ma Choong Je

机构信息

Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon 200-701, Republic of Korea.

Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon 200-701, Republic of Korea ; Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea.

出版信息

Pharmacogn Mag. 2015 Oct;11(Suppl 2):S303-7. doi: 10.4103/0973-1296.166065.

BACKGROUND

Tilia amurensis (Tiliacese) has been used for anti-tumor and anti-inflammatory in Korea, China, and Japan.

OBJECTIVE

In this study, we isolated five compounds from T. amurensis and determined whether protected neuronal cells against glutamate-induced oxidative stress in HT22 cells.

MATERIALS AND METHODS

Compounds were isolated using chromatographic techniques including silica gel, Sephadex LH-20 open column and high performance liquid chromatography analysis, and evaluated neuroprotective effect in HT22 cells by 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay.

RESULTS

β-D-fructofuranosyl α-D-glucopyranoside (1), (-)-epicatechin (2), nudiposide (3), lyoniside (4), and scopoletin (5) were isolated by bioactivity-guided fractionation from the ethyl acetate fraction of T. amurensis. Among them, (-)-epicatechin, nudiposide, lyoniside, and scopoletin had significant neuroprotective activities against glutamate-injured neurotoxicity in HT22 cells.

CONCLUSION

These results demonstrated that compound two, three, four, and five have a pronounced protective effect against glutamate-induced neurotoxicity in HT22 cells.