从徐长卿中分离得到的4,5-二甲氧基邻苯二酚对HT22细胞的神经保护作用。
Neuroprotective effects of 4,5-dimethoxypyrocatechol isolated from Cynanchum paniculatum on HT22 cells.
作者信息
Weon Jin Bae, Lee Bohyoung, Yun Bo-Ra, Lee Jiwoo, Ma Choong Je
机构信息
Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon, Korea.
Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon, Korea ; Research Institute of Biotechnology, Kangwon National University, Chuncheon, Korea.
出版信息
Pharmacogn Mag. 2014 Apr;10(38):161-4. doi: 10.4103/0973-1296.131028.
BACKGROUND
Cynanchum paniculatum, belongs to the family Asclepiadaceae and is used to treat various diseases, such as invigorate blood, alleviate edema and to relieve pain and toxicity for a long time.
MATERIALS AND METHODS
4,5-Dimethoxypyrocatechol was isolated from the 80% methanol extract of C. paniculatum and its neuroprotective effect was evaluated by MTT assay.
RESULTS
4,5-Dimethoxypyrocatechol had neuroprotective effect on the glutamate-induced cellular oxidative death in HT22 cells.
CONCLUSION
Furthermore, we found that reactive oxygen species (ROS) accumulation and calcium concentration by oxidative stress were reduced by 4,5-dimethoxypyrocatechol in HT22 cells.
背景
白首乌属于萝藦科，长期以来被用于治疗各种疾病，如活血、利水消肿以及止痛解毒。
材料与方法
从白首乌80%甲醇提取物中分离得到4,5-二甲氧基邻苯二酚，并通过MTT法评估其神经保护作用。
结果
4,5-二甲氧基邻苯二酚对HT22细胞中谷氨酸诱导的细胞氧化死亡具有神经保护作用。
结论
此外，我们发现4,5-二甲氧基邻苯二酚可减少HT22细胞中氧化应激导致的活性氧（ROS）积累和钙浓度。
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