Cabral-Santos Carolina, Gerosa-Neto José, Inoue Daniela Sayuri, Panissa Valéria Leme Gonçalves, Gobbo Luís Alberto, Zagatto Alessandro Moura, Campos Eduardo Zapaterra, Lira Fábio Santos
Exercise and Immunometabolism Research Group, Department of Physical Education, Universidade Estadual Paulista , Presidente Prudente, São Paulo, Brazil.
Department of Sports, School of Physical Education and Sports, University of São Paulo , São Paulo, Brazil.
J Sports Sci Med. 2015 Nov 24;14(4):849-56. eCollection 2015 Dec.
The purpose of this study was to compare the effect of high-intensity intermittent exercise (HIIE) versus volume matched steady state exercise (SSE) on inflammatory and metabolic responses. Eight physically active male subjects completed two experimental sessions, a 5-km run on a treadmill either continuously (70% vVO2max) or intermittently (1:1 min at vVO2max). Blood samples were collected at rest, immediately, 30 and 60 minutes after the exercise session. Blood was analyzed for glucose, non-ester fatty acid (NEFA), uric acid, lactate, cortisol, and cytokines (IL-6, IL-10 and TNF-α) levels. The lactate levels exhibited higher values immediately post-exercise than at rest (HIIE 1.34 ± 0.24 to 7.11 ± 2.85, and SSE 1.35 ± 0.14 to 4.06±1.60 mmol·L(-1), p < 0.05), but HIIE promoted higher values than SSE (p < 0.05); the NEFA levels were higher immediately post-exercise than at rest only in the SSE condition (0.71 ± 0.04 to 0.82±0.09 mEq/L, respectively, p < 0.05), yet, SSE promoted higher values than HIIE immediately after exercise (HIIE 0.72±0.03 vs SSE 0.82±0.09 mEq·L(-1), p < 0.05). Glucose and uric acid levels did not show changes under the different conditions (p > 0.05). Cortisol, IL-6, IL-10 and TNF-α levels showed time-dependent changes under the different conditions (p < 0.05), however, the area under the curve of TNF-α in the SSE were higher than HIIE (p < 0.05), and the area under the curve of IL-6 in the HIIE showed higher values than SSE (p < 0.05). In addition, both exercise conditions promote increased IL-10 levels and IL-10/TNF-α ratio (p < 0.05). In conclusion, our results demonstrated that both exercise protocols, when volume is matched, promote similar inflammatory responses, leading to an anti-inflammatory status; however, the metabolic responses are different. Key pointsMetabolic contribution of both exercise, HIIE and SSE, was different.Both protocols leading to an anti-inflammatory status.HIIE induce a higher energy expenditure take into account total session duration.
本研究的目的是比较高强度间歇运动（HIIE）与运动量匹配的稳态运动（SSE）对炎症和代谢反应的影响。八名身体活跃的男性受试者完成了两个实验阶段，即在跑步机上连续进行5公里跑步（70%最大摄氧量）或间歇进行（以最大摄氧量进行1:1分钟的运动）。在运动前休息时、运动结束后即刻、30分钟和60分钟采集血样。分析血液中的葡萄糖、非酯化脂肪酸（NEFA）、尿酸、乳酸、皮质醇和细胞因子（IL-6、IL-10和TNF-α）水平。运动后即刻的乳酸水平高于休息时（HIIE组为1.34±0.24至7.11±2.85，SSE组为1.35±0.14至4.06±1.60 mmol·L⁻¹，p<0.05），但HIIE组的乳酸水平高于SSE组（p<0.05）；仅在SSE条件下，运动后即刻的NEFA水平高于休息时（分别为0.71±0.04至0.82±0.09 mEq/L，p<0.05），然而，运动后即刻SSE组的NEFA水平高于HIIE组（HIIE组为0.72±0.03 vs SSE组为0.82±0.09 mEq·L⁻¹，p<0.05）。不同条件下葡萄糖和尿酸水平未显示变化（p>0.05）。皮质醇、IL-6、IL-10和TNF-α水平在不同条件下呈现时间依赖性变化（p<0.05），然而，SSE组TNF-α的曲线下面积高于HIIE组（p<0.05），HIIE组IL-6的曲线下面积高于SSE组（p<0.05）。此外，两种运动条件均促进IL-10水平和IL-10/TNF-α比值升高（p<0.05）。总之，我们的结果表明，当运动量匹配时，两种运动方案均促进相似的炎症反应，导致抗炎状态；然而，代谢反应不同。要点：HIIE和SSE这两种运动的代谢贡献不同。两种方案均导致抗炎状态。考虑到总运动时长，HIIE诱导更高的能量消耗。
