Kumar Brajesh, Smita Kumari, Seqqat Rachid, Benalcazar Karen, Grijalva Marcelo, Cumbal Luis
Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B, Ecuador.
Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B, Ecuador.
J Photochem Photobiol B. 2016 Jun;159:8-13. doi: 10.1016/j.jphotobiol.2016.03.011. Epub 2016 Mar 10.
In this article, biosynthesis of silver nanoparticles (AgNPs) using Andean Mora (Rubus glaucus Benth.) leaf has been reported. Different analytical techniques including UV-vis spectroscopy, dynamic light scattering (DLS), transmission electron microscopy (TEM) and X-ray diffraction (XRD) were used for the characterization of AgNPs. The initial appearance of color change with the intense surface plasmon resonance (SPR) bands around 440-455 in UV-visible spectra revealing the formation of AgNPs. The TEM image showed the AgNPs to be anisotropic, quasi-spherical in shape with sizes in the range of 12-50nm. On the other hand, XRD studies revealed the formation of face-centered cubic structure for AgNPs. The surface modified AgNPs showed no cytotoxicity at the concentration ranging from 0.01μM to 1.0μM on the Hepatic cancer (Hep-G2) cell line and observed antioxidant efficacy >70% at the concentration 0.05mM/0.20mL against 1, 1-diphenyl-2-picrylhydrazyl. From the results obtained it is suggested that AgNPs could be used effectively in future drug delivery systems and other biomedical concerns.
本文报道了利用安第斯黑莓（Rubus glaucus Benth.）叶生物合成银纳米颗粒（AgNPs）的过程。采用了包括紫外可见光谱、动态光散射（DLS）、透射电子显微镜（TEM）和X射线衍射（XRD）在内的不同分析技术对AgNPs进行表征。紫外可见光谱中在440 - 455附近出现强烈的表面等离子体共振（SPR）带且颜色发生变化，这表明AgNPs已形成。TEM图像显示AgNPs呈各向异性，形状为准球形，尺寸在12 - 50nm范围内。另一方面，XRD研究表明AgNPs形成了面心立方结构。表面修饰的AgNPs在0.01μM至1.0μM浓度范围内对肝癌（Hep - G2）细胞系无细胞毒性，并且在0.05mM/0.20mL浓度下对1,1 - 二苯基 - 2 - 苦基肼的抗氧化功效>70%。根据所得结果表明，AgNPs未来可有效地用于药物递送系统及其他生物医学领域。
