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BACKGROUND

Silver nanoparticles (Ag/Ag₂O NPs) have garnered attention for their potent antioxidant, antimicrobial, and anti-inflammatory properties, showing promise for therapeutic applications, particularly in mitigating chemical-induced toxicity.

OBJECTIVE

This study aimed to synthesize Ag/Ag₂O NPs using Olea europaea (olive) leaf extract as a green, eco-friendly reducing agent and evaluate their protective effects against metribuzin-induced toxicity in Wistar rats, focusing on oxidative stress, hematological parameters, and lipid profiles, with specific dose optimization.

METHODOLOGY

Ag/Ag₂O NPs were synthesized using Olea europaea leaf extract, and their properties were confirmed via XRD, FTIR, SEM, EDS, and UV-visible spectroscopy. Wistar rats exposed to metribuzin (110 mg/kg/day) were treated with two doses of Ag/Ag₂O NPs (0.062 mg/kg and 0.125 mg/kg). Hematological and biochemical markers were assessed to evaluate the NPs' protective effects.

RESULTS

Physicochemical characterization confirmed the successful formation of Ag/Ag₂O NPs loaded with phytochemicals, exhibiting crystallite sizes of 23 nm and 19 nm, a particle size of 25 nm, and significant peaks in XRD, FTIR, and UV-Vis spectra indicating the formation of Ag/Ag₂O. Metribuzin exposure led to significant hematological disruptions (elevated WBC, reduced RBC and hemoglobin) and worsened lipid profiles (increased cholesterol, LDL, and triglycerides). The lower NP dose (0.062 mg/kg) improved WBC, RBC, hemoglobin, and platelet counts, normalized lipid levels, and positively influenced biochemical markers such as serum creatinine and uric acid. In contrast, the higher NP dose (0.125 mg/kg) showed mixed results, with some improvements but an increase in triglycerides and continued elevation of ASAT and ALAT enzyme levels.

CONCLUSION

Ag/Ag₂O NPs synthesized via green methods using olive leaf extract effectively mitigated metribuzin-induced toxicity, especially at lower doses, by improving oxidative stress markers and hematological and biochemical profiles. Dose optimization is crucial to maximize therapeutic benefits and minimize adverse effects, underscoring their potential in treating chemical-induced toxicity.

背景

银纳米颗粒（Ag/Ag₂O NPs）因其强大的抗氧化、抗菌和抗炎特性而备受关注，在治疗应用方面显示出前景，特别是在减轻化学诱导的毒性方面。

目的

本研究旨在使用油橄榄（橄榄）叶提取物作为绿色、环保的还原剂合成Ag/Ag₂O NPs，并评估其对嗪草酮诱导的Wistar大鼠毒性的保护作用，重点关注氧化应激、血液学参数和脂质谱，并进行特定剂量优化。

方法

使用油橄榄叶提取物合成Ag/Ag₂O NPs，并通过XRD、FTIR、SEM、EDS和紫外可见光谱确认其性质。暴露于嗪草酮（110 mg/kg/天）的Wistar大鼠用两剂Ag/Ag₂O NPs（0.062 mg/kg和0.125 mg/kg）进行治疗。评估血液学和生化指标以评估纳米颗粒的保护作用。

结果

物理化学表征证实成功形成了负载植物化学物质的Ag/Ag₂O NPs，微晶尺寸为23 nm和19 nm，粒径为25 nm，XRD、FTIR和紫外可见光谱中的显著峰表明形成了Ag/Ag₂O。嗪草酮暴露导致显著的血液学紊乱（白细胞升高、红细胞和血红蛋白降低）和脂质谱恶化（胆固醇、低密度脂蛋白和甘油三酯增加）。较低的纳米颗粒剂量（0.062 mg/kg）改善了白细胞、红细胞、血红蛋白和血小板计数，使脂质水平正常化，并对血清肌酐和尿酸等生化指标产生积极影响。相比之下，较高的纳米颗粒剂量（0.125 mg/kg）显示出混合结果，有一些改善，但甘油三酯增加，ASAT和ALAT酶水平持续升高。