Marycz Krzysztof, Mierzejewska Katarzyna, Śmieszek Agnieszka, Suszynska Ewa, Malicka Iwona, Kucia Magda, Ratajczak Mariusz Z
Faculty of Biology, University of Environmental and Life Sciences, 50-375 Wroclaw, Poland ; Wroclaw Research Center EIT+, 54-066 Wroclaw, Poland.
Department of Regenerative Medicine, Medical University of Warsaw, 02-091 Warsaw, Poland.
Stem Cells Int. 2016;2016:5756901. doi: 10.1155/2016/5756901. Epub 2015 Nov 9.
Endurance exercise has been reported to increase the number of circulating hematopoietic stem/progenitor cells (HSPCs) in peripheral blood (PB) as well as in bone marrow (BM). We therefore became interested in whether endurance exercise has the same effect on very small embryonic-like stem cells (VSELs), which have been described as a population of developmentally early stem cells residing in BM. Mice were run daily for 1 hour on a treadmill for periods of 5 days or 5 weeks. Human volunteers had trained in long-distance running for one year, six times per week. FACS-based analyses and RT-PCR of murine and human VSELs and HSPCs from collected bone marrow and peripheral blood were performed. We observed that endurance exercise increased the number of VSELs circulating in PB and residing in BM. In parallel, we observed an increase in the number of HSPCs. These observations were subsequently confirmed in young athletes, who showed an increase in circulating VSELs and HSPCs after intensive running exercise. We provide for the first time evidence that endurance exercise may have beneficial effects on the expansion of developmentally early stem cells. We hypothesize that these circulating stem cells are involved in repairing minor exercise-related tissue and organ injuries.
据报道，耐力运动可增加外周血（PB）以及骨髓（BM）中循环造血干细胞/祖细胞（HSPCs）的数量。因此，我们开始关注耐力运动对极小型胚胎样干细胞（VSELs）是否有同样的影响，VSELs被描述为存在于骨髓中的一群发育早期干细胞。小鼠每天在跑步机上跑步1小时，持续5天或5周。人类志愿者进行了为期一年的长跑训练，每周6次。对采集的骨髓和外周血中的小鼠和人类VSELs及HSPCs进行了基于流式细胞术的分析和逆转录聚合酶链反应。我们观察到耐力运动增加了外周血中循环的以及骨髓中存在的VSELs数量。同时，我们观察到HSPCs数量增加。这些观察结果随后在年轻运动员中得到证实，他们在高强度跑步运动后循环VSELs和HSPCs增加。我们首次提供证据表明耐力运动可能对发育早期干细胞的扩增有有益影响。我们假设这些循环干细胞参与修复与运动相关的轻微组织和器官损伤。
