Callanan Mark C, Plummer Hillary A, Green T Meares, Opitz Tyler, Broderick Thaddeus, Rendos Nicole, Anz Adam W
Orthopedic Clinic, Shreveport, Louisiana, U.S.A.
U.S. Army Aeromedical Research Laboratory, 6901 Farrel Road, Fort Rucker, Alabama, U.S.A.
Arthrosc Sports Med Rehabil. 2022 Feb 11;4(3):e877-e882. doi: 10.1016/j.asmr.2021.12.018. eCollection 2022 Jun.
The purpose of this study was to determine the effects of blood flow restriction (BFR) using a pneumatic tourniquet on CD34 cells, platelets, white blood cells, neutrophils, lymphocytes, lactate, and glucose compared with standard exercise.
Fifteen healthy volunteers (8 males and 7 females, 28.6 ± 3.6 years old) who were able to perform the exercise sessions on a VersaClimber participated. Participants were randomized to undergo an experimental (EXP) occluded testing session using the pneumatic tourniquets on all 4 extremities and a control (CON) session. The exercise protocol concluded after 9 minutes or when participants reached a rating of perceived exertion of 20. Blood draws were performed before testing and immediately after the exercise session. Blood analysis consisted of complete blood counts as well as flow cytometry to measure peripheral CD34 counts as a marker for hematopoietic progenitor cells (HPCs).
A significant increase from before to after exercise values was observed in both the EXP and CON groups with CD34, WBC counts, platelets, and lymphocytes; however, no differences existed between EXP and CON groups for any variable. CD34 increased in the EXP (3.1 ± 1.6 vs. 4.3 ± 1.8 cells · L; < .001) and CON (3.3 ± 1.9 vs. 4.4 ± 1.4 cells · L; < .001) sessions. White blood cells also significantly increased in both the EXP (7.8 ± 1.4 vs. 11.8 ± 2.5 K · L K · L; < .001) and CON (7.5 ± 1.8 vs. 11.3 ± 3.0 K · L; < .001) sessions. Platelets also increased in both the EXP (258.6 ± 52.5 vs. 309.9 ± 52.7 K · L; < .001) and CON (263.1 ± 44.7 vs. 316.1 ± 43.9 K · L; < .001) sessions, and conversely, a significant decrease in the average neutrophil counts in the EXP (mean difference = -13.7%; < .001) and CON (mean difference = -13.2%; < .001) sessions was observed. Lymphocyte counts in the EXP (mean difference = 22.8%; < .001) and CON (mean difference = 19.3%; < .001) sessions increased significantly.
There were no significant differences in systemic cellular responses when undergoing aerobic-based exercise with and without a pneumatic tourniquet system.
2, prospective comparative study.
本研究旨在确定与标准运动相比，使用气动止血带进行血流限制（BFR）对CD34细胞、血小板、白细胞、中性粒细胞、淋巴细胞、乳酸和葡萄糖的影响。
15名能够在VersaClimber上进行运动的健康志愿者（8名男性和7名女性，年龄28.6±3.6岁）参与了研究。参与者被随机分为接受使用气动止血带对四肢进行实验性（EXP）闭塞测试的一组和对照组（CON）。运动方案在9分钟后或参与者达到自觉用力程度20时结束。在测试前和运动后立即进行采血。血液分析包括全血细胞计数以及流式细胞术，以测量外周血CD34计数作为造血祖细胞（HPCs）的标志物。
EXP组和CON组在运动前后CD34、白细胞计数、血小板和淋巴细胞的值均有显著增加；然而，两组在任何变量上均无差异。EXP组（3.1±1.6对4.3±1.8个细胞·L；P<.001）和CON组（3.3±1.9对4.4±1.4个细胞·L；P<.001）的CD34均增加。EXP组（7.8±1.4对11.8±2.5×10⁹/L；P<.001）和CON组（7.5±1.8对11.3±3.0×10⁹/L；P<.001）的白细胞也显著增加。EXP组（258.6±52.5对309.9±52.7×10⁹/L；P<.001）和CON组（263.1±44.7对316.1±43.9×10⁹/L；P<.001）的血小板也增加，相反，EXP组（平均差异=-13.7%；P<.001）和CON组（平均差异=-13.2%；P<.001）的中性粒细胞平均计数显著下降。EXP组（平均差异=
