Kober L O, Ellwart J W, Brettel H
Institut für Experimentelle Hämatologie, München, West Germany.
J Acoust Soc Am. 1989 Jul;86(1):6-7. doi: 10.1121/1.398222.
Suspended cells of a human lymphoblastic cell line were exposed to pulsed ultrasound of 775 kHz. The pulse lengths were varied between 16 and 1000 microseconds. The mark/space ratio was always kept at 1:1. Two ultrasound intensity levels were used: 3.6 and 6.4 W/cm2 spatial peak and temporal peak. After an exposure time of 5 min, cell membrane damage was measured cytometrically by a dye exclusion test. No membrane damage was observable at 16 microseconds, whereas, at pulse lengths of 1000 microseconds, about one-third of the cells were damaged.
将人淋巴母细胞系的悬浮细胞暴露于775千赫的脉冲超声中。脉冲长度在16至1000微秒之间变化。占空比始终保持在1:1。使用了两个超声强度水平：空间峰值和时间峰值分别为3.6和6.4瓦/平方厘米。暴露5分钟后，通过染料排除试验以细胞计数法测量细胞膜损伤。在16微秒时未观察到膜损伤，而在脉冲长度为1000微秒时，约三分之一的细胞受到损伤。
