Cui Yalei, Huang Tianzhi, Zhang Xiaobo
College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China
Open Biol. 2015 Dec;5(12):150126. doi: 10.1098/rsob.150126.
MicroRNAs (miRNAs) integrate with Argonaut (Ago) to create the RNA-induced silencing complex, and regulate gene expression by silencing target mRNAs. RNA editing of miRNA may affect miRNA processing, assembly of the Ago complex and target mRNA binding. However, the function of edited miRNA, assembled within the Ago complex, has not been extensively investigated. In this study, sequence analysis of the Ago complex of Marsupenaeus japonicus shrimp infected with white spot syndrome virus (WSSV) revealed that host ADAR (adenosine deaminase acting on RNA) catalysed A-to-I RNA editing of a viral miRNA (WSSV-miR-N12) at the +16 site. This editing of the non-seed sequence did not affect association of the edited miRNA with the Ago protein, but inhibited interaction between the miRNA and its target gene (wsv399). The WSSV early gene wsv399 inhibited WSSV infection. As a result, the RNA editing of miRNA caused virus latency. Our results highlight a novel example of miRNA editing in the miRNA-induced silencing complex.
微小RNA（miRNA）与AGO蛋白结合形成RNA诱导沉默复合体，并通过使靶标mRNA沉默来调控基因表达。miRNA的RNA编辑可能会影响miRNA的加工、AGO复合体的组装以及与靶标mRNA的结合。然而，AGO复合体内组装的编辑后miRNA的功能尚未得到广泛研究。在本研究中，对感染白斑综合征病毒（WSSV）的日本囊对虾AGO复合体进行序列分析，结果显示宿主ADAR（作用于RNA的腺苷脱氨酶）催化病毒miRNA（WSSV-miR-N12）在+16位点发生A到I的RNA编辑。非种子序列的这种编辑并不影响编辑后的miRNA与AGO蛋白的结合，但抑制了miRNA与其靶基因（wsv399）之间的相互作用。WSSV早期基因wsv399可抑制WSSV感染。因此，miRNA的RNA编辑导致病毒潜伏。我们的结果突出了miRNA诱导沉默复合体中miRNA编辑的一个新例子。
Zotero 插件