Department of Microbiology, Shimane University Faculty of Medicine, Izumo, Shimane, Japan.
Ningxia Medical University, Yinchuan, Ningxia, China.
Methods Mol Biol. 2021;2181:83-95. doi: 10.1007/978-1-0716-0787-9_6.
RNA is subjected to over 100 different types of chemical modifications inside the cell. These modifications have various effects on its function and expression, resulting in RNA diversity. RNA editing or conversion of adenosine to inosine (A-to-I) in a double-stranded RNA is a type of RNA modification that can introduce mutations into the precursor of microRNA (miRNA). It can also regulate miRNA processing independently of A-to-I RNA editing. This chapter outlines the role of an A-to-I RNA editing enzyme ADAR in miRNA processing and the experimental systems used to analyze the interaction between miRNAs and ADAR.
RNA 在细胞内会受到 100 多种不同类型的化学修饰。这些修饰会对其功能和表达产生各种影响，从而导致 RNA 多样性。RNA 编辑或双链 RNA 中腺苷向肌苷的转换（A-to-I）是一种 RNA 修饰类型，它可以在 miRNA 的前体中引入突变。它还可以独立于 A-to-I RNA 编辑来调节 miRNA 的加工。本章概述了 A-to-I RNA 编辑酶 ADAR 在 miRNA 加工中的作用，以及用于分析 miRNA 和 ADAR 之间相互作用的实验系统。
