Zhang Siyuan, Xin Fan, Zhang Xiaobo
College of Life Sciences, Laboratory for Marine Biology and Biotechnology of Pilot National Laboratory for Marine Science and Technology (Qingdao) and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhejiang University, Hangzhou 310058, People's Republic of China.
iScience. 2020 Dec 9;24(1):101915. doi: 10.1016/j.isci.2020.101915. eCollection 2021 Jan 22.
Viruses depend on the host metabolic machinery to complete their life cycle in the host cytoplasm. However, the key viral factors initiating the host machinery after the virus enters the cytoplasm remain unclear. Here, we found that compounds packaged in the virions of white spot syndrome virus, such as palmitic amide, could trigger the viral life cycle in the host cytoplasm. Palmitic amide promoted virus infection by enhancing host glycolysis by binding to triosephosphate isomerase to enhance its enzymatic activity. The glycolysis enhancement resulted in lactate accumulation, thereby promoting hypoxia-inducible factor 1 (HIF-1) expression. HIF-1 upregulation further enhanced glycolysis, which in turn promoted virus infection. Therefore, our study presented novel insight into the initiation of the virus life cycle in host cells.
病毒依靠宿主的代谢机制在宿主细胞质中完成其生命周期。然而，病毒进入细胞质后启动宿主机制的关键病毒因子仍不清楚。在此，我们发现包装在白斑综合征病毒粒子中的化合物，如棕榈酰胺，可在宿主细胞质中触发病毒生命周期。棕榈酰胺通过与磷酸丙糖异构酶结合以增强其酶活性来增强宿主糖酵解，从而促进病毒感染。糖酵解增强导致乳酸积累，进而促进缺氧诱导因子1（HIF-1）的表达。HIF-1的上调进一步增强糖酵解，这反过来又促进病毒感染。因此，我们的研究为宿主细胞中病毒生命周期的启动提供了新的见解。
Zotero 插件
