Cancer Res Treat. 2003 Feb;35(1):3-8. doi: 10.4143/crt.2003.35.1.3.
Alternative splicing of CD44 and aberrant levels of soluble CD44 (sCD44) protein in the serum of cancer patients has been correlated to tumor progression and metastasis. The purpose of this study was to evaluate the concentrations, and the prognostic potential of sCD44s, sCD44v5 and sCD44v6, in patients with gastric cancer.
The serum levels of sCD44s, sCD44v5 and sCD44v6 were determined quantitatively using an enzyme-linked immunosorbent assay. Serum samples were obtained from 116 patients with gastric cancer, both before and after surgery, and from 30 healthy controls.
The serum sCD44v6 levels were significantly higher in patients with gastric cancer than in the healthy controls, whereas those of sCD44s and sCD44v5 were no different. The surgical resection of the tumor resulted in a significant reduction in all the sCD44 proteins, whereas if a surgical resection was not performed the concentrations of the sCD44v5 and sCD44v6 were not reduced prior to surgery. The serum sCD44v6 levels correlated with the venous or lymphatic invasion of the tumor and lymph node metastasis. In addition, a high preoperative serum sCD44v6 level was significantly associated with poor prognosis in patients with gastric cancer.
The preoperative serum level of sCD44v6 in patients with gastric cancer was significantly higher than that in the healthy controls, and correlated with the venous or lymphatic invasion of the tumor and lymph node metastasis. In addition, a high preoperative serum sCD44v6 level was significantly associated with poor prognosis in patients with gastric cancer. These results suggest that an elevation of the serum sCD44v6 level might be used as a new predictor of tumor invasiveness, and poor prognosis, in patients with gastric cancer.
在癌症患者的血清中，CD44 的可变剪接和可溶性 CD44（sCD44）蛋白的异常水平与肿瘤的进展和转移有关。本研究的目的是评估胃癌患者血清中 sCD44s、sCD44v5 和 sCD44v6 的浓度及其预后价值。
使用酶联免疫吸附试验定量测定 sCD44s、sCD44v5 和 sCD44v6 的血清水平。采集了 116 例胃癌患者手术前后的血清样本和 30 例健康对照者的血清样本。
胃癌患者的血清 sCD44v6 水平明显高于健康对照组，而 sCD44s 和 sCD44v5 的水平则没有差异。肿瘤的手术切除导致所有 sCD44 蛋白水平显著降低，而如果不进行手术切除，则在手术前 sCD44v5 和 sCD44v6 的浓度不会降低。血清 sCD44v6 水平与肿瘤的静脉或淋巴浸润以及淋巴结转移相关。此外，术前高血清 sCD44v6 水平与胃癌患者的不良预后显著相关。
胃癌患者术前血清 sCD44v6 水平明显高于健康对照组，与肿瘤的静脉或淋巴浸润以及淋巴结转移相关。此外，术前高血清 sCD44v6 水平与胃癌患者的不良预后显著相关。这些结果表明，血清 sCD44v6 水平的升高可能可作为预测胃癌患者肿瘤侵袭性和不良预后的新指标。
