Higgins Colleen M, Bejerman Nicolas, Li Ming, James Anthony P, Dietzgen Ralf G, Pearson Michael N, Revill Peter A, Harding Robert M
Institute of Applied Ecology, School of Applied Sciences, Auckland University of Technology, Private Bag 92006, Auckland, 1142, New Zealand.
AUT Roche Diagnostic Laboratory, Auckland University of Technology, Private Bag 92006, Auckland, 1142, New Zealand.
Arch Virol. 2016 Mar;161(3):745-8. doi: 10.1007/s00705-015-2713-7. Epub 2015 Dec 21.
We report the first genome sequence of a Colocasia bobone disease-associated virus (CBDaV) derived from bobone-affected taro [Colocasia esculenta L. Schott] from Solomon Islands. The negative-strand RNA genome is 12,193 nt long, with six major open reading frames (ORFs) with the arrangement 3'-N-P-P3-M-G-L-5'. Typical of all rhabdoviruses, the 3' leader and 5' trailer sequences show complementarity to each other. Phylogenetic analysis indicated that CBDaV is a member of the genus Cytorhabdovirus, supporting previous reports of virus particles within the cytoplasm of bobone-infected taro cells. The availability of the CBDaV genome sequence now makes it possible to assess the role of this virus in bobone, and possibly alomae disease of taro and confirm that this sequence is that of Colocasia bobone disease virus (CBDV).
我们报道了从所罗门群岛受博博内病影响的芋头[Colocasia esculenta L. Schott]中分离得到的芋博博内病相关病毒(CBDaV)的首个基因组序列。负链RNA基因组长度为12,193个核苷酸,具有六个主要开放阅读框(ORF),排列顺序为3'-N-P-P3-M-G-L-5'。所有弹状病毒的典型特征是,3'前导序列和5'尾随序列相互互补。系统发育分析表明,CBDaV是细胞质弹状病毒属的成员,这支持了之前关于在受博博内病感染的芋头细胞细胞质内存在病毒粒子的报道。现在,CBDaV基因组序列的可得性使得评估该病毒在博博内病以及可能在芋头阿洛梅病中的作用成为可能,并确认该序列就是芋博博内病病毒(CBDV)的序列。