鉴定、分子特征分析及一种新型细胞弹状病毒在希腊感染南瓜作物的流行情况。
Identification, molecular characterization and prevalence of a novel cytorhabdovirus infecting zucchini crops in Greece.
机构信息
Laboratory of Plant Pathology, Faculty of Agriculture, Forestry and Natural Environment, School of Agriculture, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece.
Laboratory of Virology, Agroscope, Route de Duillier 50, 1260, Nyon, Switzerland.
出版信息
Virus Res. 2020 Oct 2;287:198095. doi: 10.1016/j.virusres.2020.198095. Epub 2020 Jul 28.
A new cytorhabdovirus was identified in zucchini (Cucurbita pepo) in Greece with the aid of high-throughput sequencing technology. The negative-sense, single-stranded genomic RNA of the new virus was determined and includes seven open reading frames in the order 3'-N-P-P3-P4-M-G-L-5' in the antigenomic orientation. Typical rhabdovirus-like particles were observed in infected leaf material. Comparative sequence analysis and phylogenetic reconstructions suggested that the described virus is a new member of the genus Cytorhabdovirus, and it was tentatively named cucurbit cytorhabdovirus 1 (CuCV1). To our knowledge CuCV1 is the first cytorhabdovirus infecting cucurbits in nature. Our surveys indicated that it occurs in a percentage of 36.7 % in zucchini crops in Greece.
借助高通量测序技术，在希腊的西葫芦（Cucurbita pepo）中鉴定出一种新型细胞弹状病毒。新型病毒的负义单链基因组 RNA 被确定，并且在抗原基因组方向上包括七个开放阅读框 3'-N-P-P3-P4-M-G-L-5'。在受感染的叶片材料中观察到典型的杆状病毒样颗粒。比较序列分析和系统发育重建表明，所描述的病毒是细胞弹状病毒属的一个新成员，它被暂时命名为葫芦科细胞弹状病毒 1（CuCV1）。据我们所知，CuCV1 是自然界中感染葫芦科植物的第一个细胞弹状病毒。我们的调查表明，它在希腊的西葫芦作物中出现的比例为 36.7%。
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