从金光菊属植物中分离到的一种假定新型细胞弹状病毒的全基因组序列
Complete genome sequence of a putative novel cytorhabdovirus isolated from Rudbeckia sp.
机构信息
Plant Quarantine Technology Center, Department of Plant Quarantine, Animal and Plant Quarantine Agency, Gimcheon, 39660, Republic of Korea.
出版信息
Arch Virol. 2022 Nov;167(11):2381-2385. doi: 10.1007/s00705-022-05556-x. Epub 2022 Aug 3.
Through high-throughput RNA sequencing, we discovered a putative new cytorhabdovirus in the seeds of Rudbeckia sp., which we have tentatively named "rudbeckia virus 1" (RudV1). Its complete 12,502-nt genomic sequence contains five open reading frames (ORFs): ORF1 (putative nucleocapsid protein, N), ORF2 (putative phosphoprotein, P), ORF3 (putative cell-to-cell movement protein, P3), ORF4 (putative matrix protein, M), and ORF5 (putative RNA-dependent RNA polymerase, L). BLASTp searches showed that ORF1, ORF3, ORF4, and ORF5 of RudV1 are most closely related to the corresponding proteins of Tagetes erecta virus 1 (a putative member of the genus Cytorhabdovirus) with 33.87% (88% query coverage), 55.98% (89% query coverage), 35.33% (94% query coverage), and 57.75% (98% query coverage) sequence identity at the amino acid level, respectively. Phylogenetic analysis and pairwise comparisons indicated that RudV1 is a novel member of the genus Cytorhabdovirus within the family Rhabdoviridae.
通过高通量 RNA 测序，我们在 Rudbeckia sp. 的种子中发现了一种假定的新细胞杆状病毒，我们暂定名为“Rudbeckia 病毒 1”（RudV1）。其完整的 12502nt 基因组序列包含五个开放阅读框（ORFs）：ORF1（假定的核衣壳蛋白，N）、ORF2（假定的磷蛋白，P）、ORF3（假定的细胞间运动蛋白，P3）、ORF4（假定的基质蛋白，M）和 ORF5（假定的 RNA 依赖性 RNA 聚合酶，L）。BLASTp 搜索表明，RudV1 的 ORF1、ORF3、ORF4 和 ORF5 与 Tagetes erecta virus 1（假定的细胞杆状病毒属成员）的相应蛋白最为密切相关，氨基酸序列同一性分别为 33.87%（88%查询覆盖率）、55.98%（89%查询覆盖率）、35.33%（94%查询覆盖率）和 57.75%（98%查询覆盖率）。系统发育分析和成对比较表明，RudV1 是 Rhabdoviridae 科细胞杆状病毒属的一个新成员。
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