样本到结果的分子传染病检测：临床意义、局限性及潜力
Sample-to-result molecular infectious disease assays: clinical implications, limitations and potential.
作者信息
Beal Stacy G, Assarzadegan Naziheh, Rand Kenneth H
机构信息
a Department of Pathology , Immunology and Laboratory Medicine, University of Florida College of Medicine , Gainesville , FL , USA.
出版信息
Expert Rev Mol Diagn. 2016;16(3):323-41. doi: 10.1586/14737159.2016.1134325. Epub 2016 Jan 11.
Abstract
Molecular infectious disease diagnostic tests have undergone major advances in the past decade and will continue to rapidly evolve. Assays have become extraordinarily simple to perform, eliminating the need for pre-analytic sample preparation and post-amplification analysis. This allows these tests to be performed in settings without sophisticated expertise in molecular biology, including locations with limited resources. Additionally, the sensitivity and specificity of these assays is superb and many offer extremely fast turn-around times. These tests have major impacts on patient care, but also have some limitations.
摘要
分子传染病诊断测试在过去十年中取得了重大进展，并将继续快速发展。检测方法已变得极其简单，无需进行分析前的样本制备和扩增后分析。这使得这些测试能够在没有分子生物学专业知识的环境中进行，包括资源有限的地区。此外，这些检测方法的灵敏度和特异性极佳，许多检测还能提供极快的周转时间。这些测试对患者护理有重大影响，但也存在一些局限性。
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