使用市售快速聚合酶链反应检测法在不到20分钟内直接检测甲型和乙型流感病毒。
Direct Detection of Influenza A and B Viruses in Less Than 20 Minutes Using a Commercially Available Rapid PCR Assay.
作者信息
Binnicker Matthew J, Espy Mark J, Irish Cole L, Vetter Emily A
机构信息
Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA
Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
出版信息
J Clin Microbiol. 2015 Jul;53(7):2353-4. doi: 10.1128/JCM.00791-15. Epub 2015 Apr 29.
Abstract
We compared an FDA-cleared rapid (<20 min) PCR assay (Cobas Liat; Roche Diagnostics) to our routine influenza A and B real-time PCR assay (Simplexa Flu A/B & RSV Direct; Focus Diagnostics) using respiratory swabs (n = 197). The Cobas Liat influenza A and B assays demonstrated sensitivities of 99.2% (123/124) and 100% (23/23), respectively, while showing a specificity of 100% for each target.
摘要
我们使用呼吸道拭子（n = 197），将一种经美国食品药品监督管理局（FDA）批准的快速（<20分钟）聚合酶链反应（PCR）检测方法（Cobas Liat；罗氏诊断公司）与我们常规的甲型和乙型流感实时PCR检测方法（Simplexa Flu A/B & RSV Direct；Focus诊断公司）进行了比较。Cobas Liat甲型和乙型流感检测方法的敏感性分别为99.2%（123/124）和100%（23/23），而对每个靶点的特异性均为100%。
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