Mandhan Prerna, Sharma Mansi, Pandey Sushmita, Chandel Neha, Chourasia Nidhi, Moun Amit, Sharma Divyani, Sukar Rubee, Singh Niyati, Mathur Shubhangi, Kotnala Aarti, Negi Neetu, Gupta Ashish, Kumar Anuj, Suresh Kumar R, Kumar Pramod, Singh Shalini
ICMR-National Institute of Cancer Prevention and Research, Noida, India.
Front Microbiol. 2022 Jun 9;13:858555. doi: 10.3389/fmicb.2022.858555. eCollection 2022.
An effective and rapid diagnosis has great importance in tackling the ongoing COVID-19 pandemic through isolation of the infected individuals to curb the transmission and initiation of specialized treatment for the disease. It has been proven that enhanced testing capacities contribute to efficiently curbing SARS-CoV-2 transmission during the initial phases of the outbreaks. RT-qPCR is considered a gold standard for the diagnosis of COVID-19. However, in resource-limited countries expenses for molecular diagnosis limits the diagnostic capacities. Here, we present interventions of two pooling strategies as 5 sample pooling (P-5) and 10 sample pooling (P-10) in a high-throughput COVID-19 diagnostic laboratory to enhance throughput and save resources and time over a period of 6 months. The diagnostic capacity was scaled-up 2.15-folds in P-5 and 1.8-fold in P-10, reagents (toward RNA extraction and RT-qPCR) were preserved at 75.24% in P-5 and 86.21% in P-10, and time saved was 6,290.93 h in P-5 and 3147.3 h in P-10.
通过隔离感染者以遏制传播并启动针对该疾病的专门治疗，有效的快速诊断对于应对当前的新冠疫情至关重要。事实证明，在疫情爆发初期提高检测能力有助于有效遏制新冠病毒的传播。逆转录定量聚合酶链反应（RT-qPCR）被认为是诊断新冠的金标准。然而，在资源有限的国家，分子诊断的费用限制了诊断能力。在此，我们展示了在一个高通量新冠诊断实验室中两种混合策略的干预措施，即5样本混合（P-5）和10样本混合（P-10），在6个月的时间里提高了通量，节省了资源和时间。P-5的诊断能力扩大了2.15倍，P-10扩大了1.8倍，P-5中用于RNA提取和RT-qPCR的试剂保存率为75.24%，P-10中为86.21%，P-5节省的时间为6290.93小时，P-10节省的时间为3147.3小时。
