黄蓝黄芩对白血病癌细胞系K562和HL-60的生长抑制及凋亡诱导作用
Growth inhibition and apoptosis induction of Scutellaria luteo-coerulea Bornm. & Sint. on leukemia cancer cell lines K562 and HL-60.
作者信息
Motaez Mahsa, Emami Seyed Ahmad, Tayarani-Najaran Zahra
机构信息
Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad, University of Medical Sciences, Mashhad, Iran.
Department of Pharmacognosy, School of Pharmacy, Mashhad, University of Medical Sciences, Mashhad, Iran.
出版信息
Avicenna J Phytomed. 2015 Nov-Dec;5(6):553-9.
OBJECTIVE
Scutellaria (Lamiaceae) has been implicated for medicinal purposes both in modern and traditional medicine. Some species of the genus Scutellaria has extensively been studied for anticancer activity. Scutellaria luteo-coerulea (S. luteo-coerulea) is one of the Iranian species of the genus Scutellaria.
MATERIALS AND METHODS
In the present study, cytotoxic and apoptogenic properties of CH2Cl2, EtOAc, n-BuOH, and H2O fractions of S. luteo-coerulea were investigated on K562. Moreover, HL-60. DNA fragmentation in apoptotic cells were determined by propidium iodide (PI) staining (sub-G1 peak).
RESULTS
Scutellaria luteo-coerulea inhibited the growth of malignant cells in a dose-dependent manner. Among solvent fractions of S. luteo-coerulea, the CH2Cl2 fraction was found to be the most cytotoxic one among others. Sub-G1 peak in flow cytometry histogram of treated cells suggested the induction of apoptosis in S. luteo-coerulea.
CONCLUSION
Scutellaria luteo-coerulea could be a novel candidate for further analytical elucidation in respect to fine major components responsible for the cytotoxic effect of the plant also clinical evaluations.
目的
黄芩（唇形科）在现代医学和传统医学中均有药用价值。黄芩属的一些物种已被广泛研究其抗癌活性。黄蓝黄芩（S. luteo - coerulea）是伊朗黄芩属的物种之一。
材料与方法
在本研究中，研究了黄蓝黄芩的二氯甲烷、乙酸乙酯、正丁醇和水提取物对K562细胞的细胞毒性和诱导凋亡特性。此外，还研究了对HL - 60细胞的作用。通过碘化丙啶（PI）染色（亚G1峰）测定凋亡细胞中的DNA片段化。
结果
黄蓝黄芩以剂量依赖性方式抑制恶性细胞的生长。在黄蓝黄芩的溶剂提取物中，发现二氯甲烷提取物的细胞毒性最强。处理后细胞流式细胞术直方图中的亚G1峰表明黄蓝黄芩诱导了细胞凋亡。
结论
黄蓝黄芩可能是一个新的候选物，可用于进一步分析确定该植物产生细胞毒性作用的主要成分，并进行临床评估。
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