Golshan Alireza, Hassanzadeh Samira, Mojdekanloo Maryam, Tayarani-Najaran Zahra
Medicinal Plants & Natural Products Research Center (MPNPRC), North Khorasan University of Medical Sciences, Bojnurd, Iran.
Pharmacological Research Centre of Medicinal Plants, School of Medicine, Mashhad, University of Medical Sciences, Mashhad, Iran.
Avicenna J Phytomed. 2016 Nov-Dec;6(6):671-677.
(Geraniaceae) has a history of use in traditional medicine in some countries including Iran. In the present study, cytotoxic and apoptogenic properties of hydro-ethanol extract of was investigated on human prostate cancer cell lines (PC3 and DU 145) and human embryonic kidney 293 (HEK293) cells.
Cells were cultured in RPMI-1640 medium supplemented with 10% FBS at 37ºC in a humidified atmosphere of 95% air and 5% CO. The root of the plant was macerated with EtOH 70%. Cytotoxic activity of ethanol extract of was assessed using alamarBlue assay after 48 hr of treatment. Apoptotic cells were stained with propidium iodide (PI) and detected by flow cytometry (sub-G1 peak).
had cytotoxic effect on malignant cells and normal HEK293 cells in a dose-dependent manner and significantly decreased the cell viability (IC values were between 199.2 and 302.9 µg/ml). increased the sub-G1 peak in flow cytometry histogram of treated PC3 cells compared to control showing the induction of apoptosis and DNA fragmentation.
Due to cytotoxic and apoptotic activity of , the plant is suggested for further phytochemical analysis and mechanistic evaluation.
香叶天竺葵科（Geraniaceae）在包括伊朗在内的一些国家有着传统医学应用历史。在本研究中，对香叶天竺葵水乙醇提取物对人前列腺癌细胞系（PC3和DU 145）以及人胚肾293（HEK293）细胞的细胞毒性和诱导凋亡特性进行了研究。
细胞在补充有10%胎牛血清的RPMI - 1640培养基中，于37℃、95%空气和5%二氧化碳的湿润环境中培养。将植物的根用70%乙醇浸渍。处理48小时后，使用alamarBlue测定法评估香叶天竺葵乙醇提取物的细胞毒性活性。用碘化丙啶（PI）对凋亡细胞进行染色，并通过流式细胞术（亚G1峰）进行检测。
香叶天竺葵对恶性细胞和正常HEK293细胞具有剂量依赖性的细胞毒性作用，并显著降低细胞活力（IC值在199.2至302.9μg/ml之间）。与对照组相比，香叶天竺葵增加了处理后PC3细胞流式细胞术直方图中的亚G1峰，表明诱导了凋亡和DNA片段化。
由于香叶天竺葵具有细胞毒性和凋亡活性，建议对该植物进行进一步的植物化学分析和作用机制评估
