Hammam H, Refai M, Bisping W, Kirpal G
Zentralbl Veterinarmed B. 1989 May;36(3):175-9. doi: 10.1111/j.1439-0450.1989.tb00588.x.
The passive haemagglutination, enzyme-linked immunosorbent assay and indirect fluorescent antibody tests were applied to study the non-specific reactions in experimentally infected guinea pigs and tuberculin positive bovines. These cross-reactions were greatly decreased after absorption of either sera with avian PPD or bovine PPD antigen with anti-avian PPD serum. The use of both absorbed sera and antigen raised the specificity of PHA and ELISA to 100%. The use of absorbed sera rendered the IFA specific in 95%. The absorption has reduced the sensitivity of ELISA, IFA and PHA by 14, 27 and 29%, respectively.
采用被动血凝试验、酶联免疫吸附测定和间接荧光抗体试验，研究实验感染豚鼠和结核菌素阳性牛的非特异性反应。用禽纯蛋白衍生物（PPD）或牛PPD抗原与抗禽PPD血清吸收任一血清后，这些交叉反应大大降低。使用吸收后的血清和抗原可将PHA和ELISA的特异性提高到100%。使用吸收后的血清使IFA的特异性达到95%。吸收分别使ELISA、IFA和PHA的敏感性降低了14%、27%和29%。
