Kimoto M, Shimahara Y, Ikai I, Wakashiro S, Ozaki N, Tatsumi Y, Tanaka A, Kamiyama Y, Yamaoka Y, Ozawa K
Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Artif Organs. 1989 Jun;13(3):204-8. doi: 10.1111/j.1525-1594.1989.tb02864.x.
The effects of a prostacyclin analog OP-41483 on energy metabolism were studied in an isolated porcine liver perfused with human blood for 8 h. OP-41483 was administered intravenously at a rate of 0.3 microgram/kg/min during the procurement and into the perfusate at a rate of 1.0 microgram/min during perfusion. Acetoacetate, beta-hydroxybutyrate, lactate, and pyruvate were measured before perfusion and at 1, 2, 3, 5, and 8 h after perfusion, from which values the ketone body ration (acetoacetate/beta-hydroxybutyrate, KBR), reflecting the redox state of liver mitochondria, was calculated. In the OP-41483 group, KBR increased rapidly from 0.34 to 0.95, 1.61, 1.51, 2.35, and 2.04, and lactate decreased rapidly from 9.81 to 6.30, 4.51, 3.22, 2.39, and 1.33 mmol/L at the respective hours after perfusion. There were significant differences after 3 h of perfusion as compared with the control group (p less than 0.05). These results suggest that administration of OP-41483 causes an increase in mitochondrial NAD+/NADH ratio (oxidized and reduced forms of free nicotinamide-adenine dinucleotides), leading to an enhancement of the metabolic capacity of the perfused liver.
在用人血灌注8小时的离体猪肝中研究了前列环素类似物OP - 41483对能量代谢的影响。在肝脏获取过程中，以0.3微克/千克/分钟的速率静脉注射OP - 41483，在灌注过程中以1.0微克/分钟的速率加入灌注液中。在灌注前以及灌注后1、2、3、5和8小时测量乙酰乙酸、β-羟基丁酸、乳酸和丙酮酸，根据这些值计算反映肝线粒体氧化还原状态的酮体比率（乙酰乙酸/β-羟基丁酸，KBR）。在OP - 41483组中，KBR在灌注后的各小时迅速从0.34增加到0.95、1.61、1.51、2.35和2.04，乳酸则从9.81迅速降至6.30、4.51、3.22、2.39和1.33毫摩尔/升。与对照组相比，灌注3小时后存在显著差异（p < 0.05）。这些结果表明，给予OP - 41483会导致线粒体NAD⁺/NADH比率（游离烟酰胺腺嘌呤二核苷酸的氧化型和还原型）增加，从而增强灌注肝脏的代谢能力。
