Jyothi Meka Naga, Ugrappa Sridevi, Velpula Nagalaxmi, Kumar Sravan, Naik Maloth Kotya, Kodangal Srikanth, Ch Lalitha, Goyal Stuti
Senior lecturer, Department of Oral Medicine and Radiology, Aditya dental college, India.
Lecturer, faculty of dentistry, Aimst Dental Institute, Aimst University, Semeling, 08100, Bedong, Malaysia.
J Dent Res Dent Clin Dent Prospects. 2015 Summer;9(3):166-74. doi: 10.15171/joddd.2015.031. Epub 2015 Sep 16.
Background and aims. Many oral squamous cell carcinomas develop from potentially malignant disorders (PMDs)which include a variety of lesions and conditions characterized by an increased risk for malignant transformation. Thisstudy evaluated the quantitative expression of EGFR in normal oral mucosa, oral leukoplakia and oral submucous fibrosis to predict the malignant risk in compliance with the intensity of staining with EGFR. Materials and methods. Thirty subjects were included in the study, consisting of 10 oral leukoplakia (OL), 10 oral submucous fibrosis (OSMF) and 10 normal oral mucosa (NOM) as the control group. Owing to the histopathological confirmation of precancerous state of tissue, 4-μm-thick sections of tissue were taken from paraffin-embedded wax blocks for immunohistochemical staining for EGFR. Results. All the control cases showed positive expression for EGFR, while 20% of oral leukoplakia and 40% of OSMF cases showed strong expression (3+), 40% of OL and 30% of OSMF cases showed weak expression (2+), and 40% of OLand 30% of OSMF cases showed poor expression (1+) compared to controls (P=0.012). Conclusion. EGFR expression levels in the premalignant lesion appear to be a sensitive factor in predicting the neoplastic potential. This suggests that EGFR may serve as a biological marker to identify high-risk subgroups and guide prophylactic therapy with chemopreventive drugs or surgical intervention to prevent progression to carcinoma. Hence, further investigations in the direction of chemopreventive trials with a larger sample size are suggested to determine its role in the head and neck tumorigenesis.
背景与目的。许多口腔鳞状细胞癌由潜在恶性疾病（PMD）发展而来，这些疾病包括多种以恶性转化风险增加为特征的病变和病症。本研究评估了表皮生长因子受体（EGFR）在正常口腔黏膜、口腔白斑及口腔黏膜下纤维化中的定量表达，以根据EGFR染色强度预测恶性风险。材料与方法。本研究纳入30名受试者，包括10例口腔白斑（OL）、10例口腔黏膜下纤维化（OSMF）以及作为对照组的10例正常口腔黏膜（NOM）。由于组织癌前状态的组织病理学确认，从石蜡包埋蜡块中切取4μm厚的组织切片用于EGFR免疫组织化学染色。结果。所有对照病例EGFR均呈阳性表达，而与对照组相比，20%的口腔白斑病例和40%的口腔黏膜下纤维化病例呈强表达（3+），40%的口腔白斑病例和30%的口腔黏膜下纤维化病例呈弱表达（2+），40%的口腔白斑病例和30%的口腔黏膜下纤维化病例呈低表达（1+）（P = 0.012）。结论。癌前病变中的EGFR表达水平似乎是预测肿瘤发生潜能的一个敏感因素。这表明EGFR可作为一种生物标志物，用于识别高危亚组，并指导使用化学预防药物或手术干预进行预防性治疗，以防止进展为癌症。因此，建议进一步开展更大样本量的化学预防试验研究，以确定其在头颈部肿瘤发生中的作用。
