Ramezani Fatemeh, Alavian Seyed Moayed, Sadeghi Ahmadreza, Khedive Abolfazl, Ghalichi Leila, Norouzi Mehdi, Karimzadeh Hadi, Malekzadeh Reza, Montazeri Ghodrat, Nejatizadeh Azim, Ziaee Masood, Abedi Farshid, Ataei Behrooz, Yaran Majid, Sayad Babak, Somi Mohamad Hosein, Sarizadeh Gholamreza, Sanei-Moghaddam Ismail, Mansour-Ghanaei Fariborz, Rafatpanah Houshang, Keyvani Hossein, Kalantari Ebrahim, Saberfiroozi Mehdi, Rezaee Reza, Daram Maryam, Mahabadi Mostafa, Goodarzi Zahra, Poortahmasebi Vahdat, Geravand Babak, Khamseh Azam, Mahmoodi Masoud, Jazayeri Seyed Mohammad
Hepatitis B Molecular Laboratory-Department of Virology-School of Public Health-Tehran University of Medical Sciences, Tehran, Iran.
Middle East Liver Diseases Center (MELD Centers), Tehran, Iran.
Iran J Microbiol. 2015 Aug;7(4):214-20.
Iranian chronic HBV carrier's population has shown a unique pattern of genotype D distribution all around the country. The aim of this study was to explore more details of evolutionary history of carriers based on structural surface proteins from different provinces.
Sera obtained from 360 isolates from 12 Different regions of country were used for amplification and sequencing of surface proteins. A detailed mutational analysis was undertaken.
The total ratio for Missense/Silent nucleotide substitutions was 0.96. Sistan and Kermanshah showed the lowest rate of evolution between provinces (P = 0.055). On the other hand, Khorasan Razavi and Khoozestan contained the highest ratio (P = 0.055). The rest of regions were laid between these two extremes. Azarbayjan and Guilan showed the highest proportion of immune epitope distribution (91.3% and 96%, respectively). Conversely, Sistan and Tehran harbored the least percentage (66.6% and 68.8%, respectively). Kermanshah province contained only 5.2%, whereas Isfahan had 54.5% of B cell epitope distribution. In terms of T helper epitopes, all provinces showed a somehow homogeneity: 22.58% (Fars) to 46.6% (Khuzestan). On the other hand, distribution of substitutions within the CTL epitopes showed a wide range of variation between 6.6% (Khuzestan) and 63% (Kermanshah).
Further to low selection pressure found in Iranian population, the variations between different regions designate random genetic drift within the surface proteins. These finding would have some applications in terms of specific antiviral regimen, design of more efficient vaccine and public health issues.
伊朗慢性乙肝病毒携带者群体在全国呈现出独特的D基因型分布模式。本研究旨在基于来自不同省份的结构表面蛋白，探索携带者进化史的更多细节。
从该国12个不同地区的360株分离株中获取血清，用于表面蛋白的扩增和测序。进行了详细的突变分析。
错义/沉默核苷酸替换的总比例为0.96。锡斯坦和克尔曼沙阿在各省之间显示出最低的进化速率（P = 0.055）。另一方面，霍拉桑拉扎维和胡齐斯坦的比例最高（P = 0.055）。其他地区则介于这两个极端之间。东阿塞拜疆和吉兰显示出免疫表位分布的最高比例（分别为91.3%和96%）。相反，锡斯坦和德黑兰的比例最低（分别为66.6%和68.8%）。克尔曼沙阿省仅含有5.2%，而伊斯法罕的B细胞表位分布为54.5%。就辅助性T细胞表位而言，所有省份都呈现出一定程度的同质性：从22.58%（法尔斯）到46.6%（胡齐斯坦）。另一方面，CTL表位内替换的分布在6.6%（胡齐斯坦）和63%（克尔曼沙阿）之间呈现出广泛的差异。
除了在伊朗人群中发现的低选择压力外，不同地区之间的差异表明表面蛋白内存在随机遗传漂变。这些发现将在特定抗病毒方案、更有效疫苗的设计以及公共卫生问题方面具有一些应用。
