Mousset Mathilde, Flaven Elodie, Justy Fabienne, Pouzadoux Juliette, Gode Cécile, Pauwels Maxime, Gonneau Cédric
Institut des Sciences de l'Évolution, Université de Montpellier, CNRS, IRD, EPHE, CC 065, Place Eugène Bataillon 34095, Montpellier CEDEX 05, France.
Université Lille, CNRS, UMR 8198, Evo-Eco-Paleo, F-59000 Lille, France.
Appl Plant Sci. 2015 Dec 9;3(12). doi: 10.3732/apps.1500052. eCollection 2015 Dec.
Multiplexed microsatellite markers were developed for population genetic studies in the pseudometallophyte Noccaea caerulescens (Brassicaceae), a model species to investigate metal tolerance and hyperaccumulation in higher plants.
Microsatellite loci were isolated through pyrosequencing of an enriched DNA library. Three multiplexes combining four previously published and 17 newly designed markers were developed. The new markers were screened in metallicolous and nonmetallicolous populations from southern France. The total number of alleles per locus ranged from five to 18. The observed heterozygosity per locus and per population ranged from 0 to 0.83, and expected heterozygosity ranged from 0 to 0.89.
The investigated loci showed reasonable to high levels of polymorphism at the regional scale. The multiplex set should be helpful in investigating genetic diversity, population structure, and demographic history in N. caerulescens at various spatial scales.
开发了多重微卫星标记，用于对假金属植物天蓝遏蓝菜（十字花科）进行群体遗传学研究，该植物是研究高等植物金属耐受性和超积累的模式物种。
通过对富集DNA文库进行焦磷酸测序分离微卫星位点。开发了三个多重组合，包含四个先前发表的标记和17个新设计的标记。在法国南部的金属矿区和非金属矿区群体中对新标记进行了筛选。每个位点的等位基因总数在5到18个之间。每个位点和每个群体观察到的杂合度在0到0.83之间，预期杂合度在0到0.89之间。
在所研究的区域尺度上，这些位点显示出合理到较高水平的多态性。该多重组合将有助于在不同空间尺度上研究天蓝遏蓝菜的遗传多样性、群体结构和种群历史。
