Yamamoto Johtaro, Oura Makoto, Yamashita Taro, Miki Shigehito, Jin Takashi, Haraguchi Tokuko, Hiraoka Yasushi, Terai Hirotaka, Kinjo Masataka
Opt Express. 2015 Dec 14;23(25):32633-42. doi: 10.1364/OE.23.032633.
Conventional polarization-dependent fluorescence correlation spectroscopy (pol-FCS) requires two sets of photon detectors to eliminate after-pulse noises (dual-channel pol-FCS; DC-pol-FCS) in the sub-microsecond range. In this study, we successfully realized pol-FCS using a visible-wavelength superconductive nanowire single-photon detector (single-channel pol-FCS; SC-pol-FCS). The detector used is free of after-pulse noises and thus eliminates the need for dual channels in pol-FCS. Further, the optics in the SC-pol-FCS system are easier to adjust than those in the conventional system. Consequently, we obtained higher signal-to-noise ratios compared with conventional DC-pol-FCS systems. Thus, SC-pol-FCS is a potentially useful system for obtaining pol-FCS measurements, and can facilitate improved rotational diffusion studies.
传统的偏振相关荧光关联光谱法（pol-FCS）需要两组光子探测器来消除亚微秒范围内的后脉冲噪声（双通道pol-FCS；DC-pol-FCS）。在本研究中，我们使用可见波长超导纳米线单光子探测器成功实现了pol-FCS（单通道pol-FCS；SC-pol-FCS）。所使用的探测器没有后脉冲噪声，因此无需在pol-FCS中使用双通道。此外，SC-pol-FCS系统中的光学器件比传统系统中的更容易调整。因此，与传统的DC-pol-FCS系统相比，我们获得了更高的信噪比。因此，SC-pol-FCS是一种用于获得pol-FCS测量值的潜在有用系统，并且可以促进改进的旋转扩散研究。
