Peng Wan, Lu Dan-Qi, Li Gao-Fei, Zhang Xu, Yao Mi, Zhang Yong, Lin Hao-Ran
State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, College of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, College of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
Mol Immunol. 2016 Feb;70:34-46. doi: 10.1016/j.molimm.2015.12.004. Epub 2015 Dec 14.
Interferon gamma (IFNγ) is a Th1 cytokine that plays a very important role in almost all phases of immune and inflammatory responses. In this study, we explored the functions of IFNγ1 and IFNγ2 of Tetraodon nigroviridis. Treating T. nigroviridis spleen and head kidney cells in vitro with recombinant T. nigroviridis IFNγ1 protein (rTn IFNγ1) or recombinant T. nigroviridis IFNγ2 protein (rTn IFNγ2) enhanced their nitric oxide responses. Both rTn IFNγ1 and rTn IFNγ2 also induced the expression of interferon-stimulated gene 15 (ISG15), a common anti-viral gene, although the expression of the interferon-inducible Mx gene was markedly inhibited by rTn IFNγ1 and was induced by rTn IFNγ2. The in vivo effects of rTn IFNγ1 and rTn IFNγ2 on Vibrio parahaemolyticus (V. parahaemolyticus) infection were assessed by intraperitoneally injecting rTn IFNγ1 or rTn IFNγ2 (100 ng) and V. parahaemolyticus (8 × 10(10)CFU/mL) into T. nigroviridis. A comparison of the group treated only with V. parahaemolyticus and those also treated with rTn IFNγ1 or rTn IFNγ2 showed that neither of these IFNγs protected T. nigroviridis from V. parahaemolyticus infection. However, rTn IFNγ1 more rapidly and robustly promoted inflammatory responses compared with rTn IFNγ2, whereas rTn IFNγ2 was involved in inducing the host to develop a more effective response earlier during the later stage of a V. parahaemolyticus infection. Moreover, microRNA-29b (miR-29b) expression is inversely correlated with IFNγ2 expression in T. nigroviridis.
干扰素γ（IFNγ）是一种Th1细胞因子，在免疫和炎症反应的几乎所有阶段都发挥着非常重要的作用。在本研究中，我们探究了黑青斑河豚IFNγ1和IFNγ2的功能。用重组黑青斑河豚IFNγ1蛋白（rTn IFNγ1）或重组黑青斑河豚IFNγ2蛋白（rTn IFNγ2）体外处理黑青斑河豚的脾脏和头肾细胞，增强了它们的一氧化氮反应。rTn IFNγ1和rTn IFNγ2均诱导了干扰素刺激基因15（ISG15）的表达，ISG15是一种常见的抗病毒基因，尽管干扰素诱导的Mx基因的表达被rTn IFNγ1显著抑制，而被rTn IFNγ2诱导。通过向黑青斑河豚腹腔注射rTn IFNγ1或rTn IFNγ2（100 ng）和副溶血性弧菌（8×10¹⁰CFU/mL），评估rTn IFNγ1和rTn IFNγ2对副溶血性弧菌感染的体内作用。仅用副溶血性弧菌处理的组与同时用rTn IFNγ1或rTn IFNγ2处理的组的比较表明，这些IFNγ均不能保护黑青斑河豚免受副溶血性弧菌感染。然而，与rTn IFNγ2相比，rTn IFNγ1能更快速、强劲地促进炎症反应，而rTn IFNγ2在副溶血性弧菌感染后期更早地参与诱导宿主产生更有效的反应。此外，在黑青斑河豚中，微小RNA - 29b（miR - 29b）的表达与IFNγ2的表达呈负相关。
