Thewes Nicolas, Loskill Peter, Spengler Christian, Hümbert Sebastian, Bischoff Markus, Jacobs Karin
Experimental Physics, Campus E2 9, Saarland University, D-66123, Saarbrücken, Germany.
Institute of Medical Microbiology and Hygiene, Saarland University, D-66421, Homburg/Saar, Germany.
Eur Phys J E Soft Matter. 2015 Dec;38(12):140. doi: 10.1140/epje/i2015-15140-2. Epub 2015 Dec 28.
The atomic force microscope (AFM) evolved as a standard device in modern microbiological research. However, its capability as a sophisticated force sensor is not used to its full capacity. The AFM turns into a unique tool for quantitative adhesion research in bacteriology by using "bacterial probes". Thereby, bacterial probes are AFM cantilevers that provide a single bacterium or a cluster of bacteria as the contact-forming object. We present a step-by-step protocol for preparing bacterial probes, performing force spectroscopy experiments and processing force spectroscopy data. Additionally, we provide a general insight into the field of bacterial cell force spectroscopy.
原子力显微镜（AFM）已发展成为现代微生物学研究中的标准设备。然而，其作为精密力传感器的能力尚未得到充分利用。通过使用“细菌探针”，AFM成为细菌学中定量粘附研究的独特工具。因此，细菌探针是AFM悬臂，它提供单个细菌或一群细菌作为形成接触的物体。我们提出了一个制备细菌探针、进行力谱实验和处理力谱数据的分步方案。此外，我们还对细菌细胞力谱领域进行了总体介绍。
