Mackay Joanna L, Kumar Sanjay
Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA, USA.
Methods Mol Biol. 2013;931:313-29. doi: 10.1007/978-1-62703-056-4_15.
Atomic force microscopy (AFM) is a powerful and versatile tool for probing the mechanical properties of biological samples. This chapter describes the procedures for using AFM indentation to measure the elastic moduli of living cells. We include step-by-step instructions for cantilever calibration and data acquisition using a combined AFM/optical microscope system, as well as a detailed protocol for data analysis. Our protocol is written specifically for the BioScope™ Catalyst™ AFM system (Bruker AXS Inc.); however, most of the general concepts can be readily translated to other commercial systems.
原子力显微镜（AFM）是一种用于探测生物样品力学性质的强大且多功能的工具。本章描述了使用AFM压痕测量活细胞弹性模量的程序。我们提供了使用AFM/光学显微镜组合系统进行悬臂校准和数据采集的逐步说明，以及详细的数据分析方案。我们的方案是专门为BioScope™ Catalyst™ AFM系统（布鲁克AXS公司）编写的；然而，大多数一般概念可以很容易地应用于其他商业系统。
