Joshi Amaraja, Thite Sonia, Kulkarni Girish, Dhotre Dhiraj, Joseph Neetha, Venkata Ramana V, Polkade Ashish, Shouche Yogesh
Microbial Culture Collection, National Centre for Cell Science, Sai Trinity Complex, Sus Road, Pashan, Pune- 411021, India.
Int J Syst Evol Microbiol. 2016 Mar;66(3):1254-1259. doi: 10.1099/ijsem.0.000868. Epub 2015 Dec 23.
Two Gram-stain-negative, aerobic, alkaliphilic bacteria (strains MEB087T and MEB142) were isolated from sediment and water samples, respectively, collected from the alkaline Lonar Lake in Maharashtra, India. Strains MEB087T and MEB142 shared 99.8 % 16S rRNA gene sequence similarity and were 85 % related on the basis of DNA-DNA hybridization. The 16S rRNA gene sequences of both strains showed close relationship with the genus Nitrincola, and their closest neighbour was Nitrincola lacisaponensis 4CAT with 97.7 % sequence similarity. MEB087T and MEB142 exhibited only 45 % and 54 % DNA-DNA relatedness, respectively, with Nitrincola lacisaponensis DSM 16316T. Both strains were asporogenous, short, non-motile rods capable of utilizing a limited range of organic acids as sole carbon and energy sources. They were oxidase- and catalase-positive, able to reduce nitrate and nitrite; but unable to degrade DNA, urea, gelatin, casein or starch. They grew optimally at pH 9.5 (tolerating up to pH 11) and could withstand up to 0.6 M NaCl. The predominant cellular fatty acids were summed feature 8 comprising C18 : 1ω7c/C18 : 1ω6c (47-49 %) followed by summed feature 3 comprising C16 : 1ω7c/C16 : 1ω6c (28-32 %). The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The DNA G+C content was 49.3-49.7 mol%. On the basis of the phylogenetic analysis and chemotaxonomic characteristics, strains MEB087T and MEB142 represent a novel species in the genus Nitrincola, for which the name Nitrincola alkalisediminis sp. nov. is proposed. The type strain is MEB087T ( = KCTC 42948T = JCM 19317T) with MEB142 ( = KCTC 42949 = JCM 19318) as an additional strain.
从印度马哈拉施特拉邦碱性的洛纳尔湖采集的沉积物和水样中，分别分离出两株革兰氏阴性、需氧、嗜碱细菌（菌株MEB087T和MEB142）。菌株MEB087T和MEB142的16S rRNA基因序列相似度为99.8%，基于DNA-DNA杂交分析，二者的亲缘关系为85%。两株菌的16S rRNA基因序列均显示与硝化球菌属关系密切，其最接近的亲缘种是乳酸硝化球菌4CAT，序列相似度为97.7%。MEB087T和MEB142与乳酸硝化球菌DSM 16316T的DNA-DNA相关性分别仅为45%和54%。两株菌均不产芽孢，为短杆状、无运动性，能够利用有限种类的有机酸作为唯一碳源和能源。它们氧化酶和过氧化氢酶呈阳性，能够还原硝酸盐和亚硝酸盐；但不能降解DNA、尿素、明胶、酪蛋白或淀粉。它们在pH 9.5时生长最佳（可耐受pH 11），能耐受高达0.6 M的NaCl。主要的细胞脂肪酸为特征性总和8，包括C18 : 1ω7c/C18 : 1ω6c（47 - 49%），其次是特征性总和3，包括C16 : 1ω7c/C16 : 1ω6c（28 - 32%）。主要的极性脂为双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰胆碱。DNA的G+C含量为49.3 - 49.7 mol%。基于系统发育分析和化学分类特征，菌株MEB087T和MEB142代表硝化球菌属中的一个新物种，为此提出新名称碱减量硝化球菌（Nitrincola alkalisediminis sp. nov.）。模式菌株为MEB087T（=KCTC 42948T =JCM 19317T），MEB142（=KCTC 42949 =JCM 19318）作为另一菌株。
