Wang Qingyu, Xiao Yi, Gao Danning, Su Longyi, Yan Yongliang, Zhan Yuhua, Zhou Zhengfu, Ke Xiubin
Biotechnology Research Institute/Key Laboratory of Agricultural Microbiome (MARA), Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Int J Syst Evol Microbiol. 2025 Aug;75(8). doi: 10.1099/ijsem.0.006894.
A Gram-stain-negative, aerobic, rod-shaped, oxidase- and catalase-positive and non-motile bacterium, named MINF-07-Sa-05, was isolated from sediment samples collected from Niya Fish Salt Lake in Xinjiang, northwest China. Phylogenetic tree based on 16S rRNA gene sequences showed that strain MINF-07-Sa-05 consistently fell within the genus and formed a clade together with ZV-19 (98.97%) and 4CA (96.16%). Genomic average nucleotide identity values for strain MINF-07-Sa-05 with the type strains within the genus ranged from 69.8 to 87.8%, while the DNA-DNA hybridization values for strain MINF-07-Sa-05 with these strains ranged from 20.4 to 33.7%. The genome sequence size of the strain was 4.81 Mb, with a DNA G+C content of 52.6 mol%. Its growth occurred at 4-40 °C, at pH 6.0-10.0, and tolerated up to 9% (w/v) NaCl. The major fatty acids contained summed feature 8 (C 7/C 6; 59.8%), summed feature 3 (C 7/C 6; 20.4%) and C (13.8%). The primary respiratory quinone was ubiquinone-8. The main polar lipids were phosphatidylglycerol and phosphatidylethanolamine. Genomic analyses showed a high abundance of genes involved in the nitrogen, sulphur cycle and antibiotic resistance. According to the genotypic, phylogenetic and chemotaxonomic data, strain MINF-07-Sa-05 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is MINF-07-Sa-05 (=GDMCC 1.4420=JCM 37233).
从中国西北部新疆尼雅鱼盐湖采集的沉积物样本中分离出一株革兰氏阴性、需氧、杆状、氧化酶和过氧化氢酶阳性且不运动的细菌，命名为MINF-07-Sa-05。基于16S rRNA基因序列构建的系统发育树表明，菌株MINF-07-Sa-05始终属于该属，并与ZV-19（98.97%）和4CA（96.16%）形成一个分支。菌株MINF-07-Sa-05与该属内模式菌株的基因组平均核苷酸同一性值在69.8%至87.8%之间，而其与这些菌株的DNA-DNA杂交值在20.4%至33.7%之间。该菌株的基因组序列大小为4.81 Mb，DNA G+C含量为52.6 mol%。其生长温度范围为4-40℃，pH值范围为6.0-10.0，可耐受高达9%（w/v）的NaCl。主要脂肪酸包括总和特征8（C18:1ω7c/C18:1ω6c；59.8%）、总和特征3（C16:1ω7c/C16:1ω6c；20.4%）和C16:0（13.8%）。主要呼吸醌为泛醌-8。主要极性脂为磷脂酰甘油和磷脂酰乙醇胺。基因组分析显示参与氮、硫循环和抗生素抗性的基因丰度很高。根据基因型、系统发育和化学分类数据，菌株MINF-07-Sa-05代表该属内的一个新物种，为此提出新物种名称sp. nov.。模式菌株为MINF-07-Sa-05（=GDMCC 1.4420=JCM 37233）。
