中国橄榄（Canarium album L.）果实不同溶剂提取物的抗氧化和抗糖化性质。

Antioxidant and antiglycation properties of different solvent extracts from Chinese olive (Canarium album L.) fruit.

机构信息

Department of Food Science and Technology, Central Taiwan University of Science and Technology, Taichung, Taiwan, ROC; Department of Bioindustry Technology, Da-Yeh University, Changhua, Taiwan, ROC.

Department of Food Science and Technology, Central Taiwan University of Science and Technology, Taichung, Taiwan, ROC.

出版信息

Asian Pac J Trop Med. 2015 Dec;8(12):1013-1021. doi: 10.1016/j.apjtm.2015.11.013. Epub 2015 Nov 14.

DOI:10.1016/j.apjtm.2015.11.013

PMID:26706672

Abstract

OBJECTIVE

To evaluate the antioxidant activity, antiglycation property, and bioactive components content of different solvent extracts from Chinese olive (Canarium album L.) fruit.

METHODS

The dry powder of Chinese olive fruit was extracted with different solvents, i.e., water, water/ethanol (1/1, v/v), ethanol, methanol, acetone and ethyl acetate. The total phenolic, total flavonoids and total triterpenoids contents of various extracts were determined by spectrophotometric methods. Phenolic compounds were identified by high performance liquid chromatography. The assayed antioxidant activity was determined in vitro models such as antioxidant capacity by radical scavenging activity using 2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), 1,1-diphenyl-2-picryl- hydrazyl (DPPH) and nitrite oxide methods, chelating activity on metal ions, lipid and protein peroxidation methods. In vitro glucose-bovine serum albumin assay was used to evaluate the antiglycation of various extracts.

RESULTS

The water/ethanol extracts of Chinese olive fruit exerted significant scavenging effects on free radicals and strong inhibitory effects on advanced glycation end products formation. The Chinese olive fruit extracts were rich in phenolic compounds and triterpenoids. Gallic acid, ferulic acid and rutin were identified from the water/ethanol extracts. Correlation analysis indicated that there was a linear relationship between the antioxidant potency, free radical scavenging ability and phenolic compounds content of the Chinese olive fruit extracts.

CONCLUSIONS

Chinese olive fruit could be a natural candidate for studies of dietary complement to diabetes treatment since it combines antioxidant and antiglycation activities.

摘要

目的

评估不同溶剂从橄榄（Canarium album L.）果实提取物的抗氧化活性、抗糖化性质和生物活性成分含量。

方法

用不同溶剂，即水、水/乙醇（1/1，v/v）、乙醇、甲醇、丙酮和乙酸乙酯，对橄榄果实干粉进行提取。用分光光度法测定各种提取物的总酚、总黄酮和总三萜含量。用高效液相色谱法鉴定酚类化合物。用抗氧化能力测定法，如使用 2,2'-偶氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）、1,1-二苯基-2-苦基肼（DPPH）和亚硝酸盐氧化物法、金属离子螯合活性、脂质和蛋白质过氧化法等体外模型来测定抗氧化活性。体外葡萄糖-牛血清白蛋白测定法用于评价各种提取物的抗糖化作用。