State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, People's Republic of China.
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, People's Republic of China.
Bioresour Technol. 2016 Feb;202:152-7. doi: 10.1016/j.biortech.2015.12.002. Epub 2015 Dec 14.
In this study, Actinobacillus succinogenes NJ113 microbial electrolysis cells (MECs) were used to enhance the reducing power responsible for succinic acid production from corncob hydrolysate. During corncob hydrolysate fermentation, electric MECs resulted in a 1.31-fold increase in succinic acid production and a 1.33-fold increase in the reducing power compared with those in non-electric MECs. When the hydrolysate was detoxified by combining Ca(OH)2, NaOH, and activated carbon, succinic acid production increased from 3.47 to 6.95 g/l. Using a constant potential of -1.8 V further increased succinic acid production to 7.18 g/l. A total of 18.09 g/l of succinic acid and a yield of 0.60 g/g total sugar were obtained after a 60-h fermentation when NaOH was used as a pH regulator. The improved succinic acid yield from corncob hydrolysate fermentation using A. succinogenes NJ113 in electric MECs demonstrates the great potential of using biomass as a feedstock to cost-effectively produce succinate.
在这项研究中，使用 Actinobacillus succinogenes NJ113 微生物电解池 (MEC) 来增强负责从玉米芯水解物生产琥珀酸的还原能力。在玉米芯水解物发酵过程中，与非电动 MEC 相比，电动 MEC 使琥珀酸产量增加了 1.31 倍，还原能力增加了 1.33 倍。当将 Ca(OH)2、NaOH 和活性炭结合起来对水解物进行解毒时，琥珀酸产量从 3.47 增加到 6.95 g/l。当使用-1.8 V 的恒定电势时，琥珀酸产量进一步增加到 7.18 g/l。当使用 NaOH 作为 pH 调节剂时，经过 60 小时发酵，总共获得了 18.09 g/l 的琥珀酸和 0.60 g/g 总糖的产率。在电动 MEC 中使用 A. succinogenes NJ113 从玉米芯水解物发酵中提高琥珀酸产量，证明了使用生物质作为原料来经济有效地生产琥珀酸盐的巨大潜力。
