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利用 Actinobacillus succinogenes 将农作物秸秆废弃物高效转化为琥珀酸生产。

Efficient conversion of crop stalk wastes into succinic acid production by Actinobacillus succinogenes.

机构信息

National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100190, PR China.

出版信息

Bioresour Technol. 2010 May;101(9):3292-4. doi: 10.1016/j.biortech.2009.12.064. Epub 2010 Jan 12.

Abstract

Succinic acid is valued as a key platform chemical for use in a variety of synthetic applications. Efficient biosynthesis of succinic acid from renewable biomass resource is reported in this paper. Batch fermentations were carried out to analyze influence of several carbon sources on succinic acid production from feedstock wastes by Actinobacillus succinogenes BE-1. Crop stalk wastes, including corn stalk and cotton stalk, were enzymatically converted into a carbohydrate-rich feedstock, obtaining glucose concentrations approaching 65-80% of the total reducing sugar. For the anaerobic batch cultivation with cotton stalk hydrolysates, the production of succinic acid was 15.8 g l(-1) with a high yield of 1.23 g per g glucose. Glucose and xylose were utilized at same time, while cellubiose was not consumed until glucose and xylose were completely consumed.

摘要

琥珀酸作为一种关键的平台化学品,可用于各种合成应用。本文报道了从可再生生物质资源中高效合成琥珀酸。通过发酵实验,分析了不同碳源对发酵法生产琥珀酸的影响。利用作物秸秆,包括玉米秸秆和棉花秸秆,经酶解转化为富含碳水化合物的原料,得到的总还原糖中葡萄糖浓度接近 65-80%。在以棉秆水解液为原料的厌氧分批培养中,琥珀酸的产量为 15.8 g/L,葡萄糖得率为 1.23 g/g。葡萄糖和木糖同时被利用,而当葡萄糖和木糖完全消耗后,纤维素才被消耗。

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