Tabynov Kaissar, Yespembetov Bolat, Ryskeldinova Sholpan, Zinina Nadezhda, Kydyrbayev Zhailaubay, Kozhamkulov Yerken, Inkarbekov Dulat, Sansyzbay Abylai
The Research Institute for Biological Safety Problems, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeiskiy 080409, Kazakhstan.
The Research Institute for Biological Safety Problems, Zhambulskaya oblast, Kordaiskiy rayon, Gvardeiskiy 080409, Kazakhstan.
Vaccine. 2016 Jan 20;34(4):438-444. doi: 10.1016/j.vaccine.2015.12.028. Epub 2015 Dec 19.
This study analyzed the duration of the antigen-specific humoral and T-cell immune responses and protectiveness of a recently-developed influenza viral vector Brucella abortus (Flu-BA) vaccine expressing Brucella proteins Omp16 and L7/L12 and containing the adjuvant Montadine Gel01 in cattle. At 1 month post-booster vaccination (BV), both humoral (up to 3 months post-BV; GMT IgG ELISA titer 214±55 to 857±136, with a prevalence of IgG2a over IgG1 isotype antibodies) and T-cell immune responses were observed in vaccinated heifers (n=35) compared to control animals (n=35, injected with adjuvant/PBS only). A pronounced T-cell immune response was induced and maintained for 12 months post-BV, as indicated by the lymphocyte stimulation index (2.7±0.4 to 10.1±0.9 cpm) and production of IFN-γ (13.7±1.7 to 40.0±3.0 ng/ml) at 3, 6, 9, and 12 months post-BV. Prime-boost vaccination provided significant protection against B. abortus infection at 3, 6, 9 and 12 months (study duration) post-BV (7 heifers per time point; alpha=0.03-0.01 vs. control group). Between 57.1 and 71.4% of vaccinated animals showed no signs of B. abortus infection (or Brucella isolation) at 3, 6, 9 and 12 months post-BV; the severity of infection, as indicated by the index of infection (P=0.0003 to <0.0001) and rates of Brucella colonization (P=0.03 to <0.0001), was significantly lower for vaccinated diseased animals than appropriate control animals. Good protection from B. abortus infection was also observed among pregnant vaccinated heifers (alpha=0.03), as well as their fetuses and calves (alpha=0.01), for 12 months post-BV. Additionally, 71.4% of vaccinated heifers calved successfully whereas all pregnant control animals aborted (alpha=0.01). Prime-boost vaccination of cattle with Flu-BA induces an antigen-specific humoral and pronounced T cell immune response and most importantly provides good protectiveness, even in pregnant heifers, for at least 12 months post-BV.
本研究分析了一种最近研制的表达布鲁氏菌蛋白Omp16和L7/L12并含有佐剂Montadine Gel01的流感病毒载体牛流产布鲁氏菌（Flu-BA）疫苗在牛体内的抗原特异性体液免疫和T细胞免疫反应的持续时间以及保护效果。在加强免疫接种（BV）后1个月，与对照动物（n = 35，仅注射佐剂/ PBS）相比，接种疫苗的小母牛（n = 35）中观察到了体液免疫（BV后长达3个月；GMT IgG ELISA滴度为214±55至857±136，IgG2a同型抗体的流行率高于IgG1）和T细胞免疫反应。BV后诱导了明显的T细胞免疫反应并维持了12个月，这通过BV后3、6、9和12个月时的淋巴细胞刺激指数（2.7±0.4至10.1±0.9 cpm）和IFN-γ的产生（13.7±1.7至40.0±3.0 ng/ml）得以体现。初免-加强免疫接种在BV后3、6、9和12个月（研究持续时间）对牛流产布鲁氏菌感染提供了显著保护（每个时间点7头小母牛；与对照组相比，α = 0.03 - 0.01）。在BV后3、6、9和12个月，57.1%至71.4%的接种疫苗动物未表现出牛流产布鲁氏菌感染（或布鲁氏菌分离）的迹象；接种疫苗的患病动物的感染严重程度，以感染指数（P = 0.0003至<0.0001）和布鲁氏菌定植率（P = 0.03至<0.0001）表示，明显低于相应的对照动物。在BV后12个月，在怀孕的接种疫苗小母牛（α = 0.03）及其胎儿和小牛（α = 0.01）中也观察到了对牛流产布鲁氏菌感染的良好保护。此外，71.4%的接种疫苗小母牛成功产犊，而所有怀孕的对照动物均流产（α = 0.01）。用Flu-BA对牛进行初免-加强免疫接种可诱导抗原特异性体液免疫和明显的T细胞免疫反应，最重要的是，即使在怀孕小母牛中，在BV后至少12个月也能提供良好的保护作用。
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