Wang Hongshu, Liu Feng, Wang Jing, Chen Nansheng
National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan, Zhejiang, China.
CAS Key Laboratory of Marine Ecology and Environmental Sciences (KLMEES), Institute of Oceanology, Chinese Academy of Sciences (IOCAS), Qingdao, Shandong, China.
Mitochondrial DNA B Resour. 2021 Sep 24;6(10):3052-3054. doi: 10.1080/23802359.2021.1978889. eCollection 2021.
Linnaeus 1753 (Ulvophyceae, Chlorophyta) is a marine green macroalga that is distributed on coasts of the Yellow Sea and the Bohai Sea in China. Here, the complete chloroplast genome of was constructed and analyzed comparatively. The chloroplast genome of is a 99,041-bp circular molecule that harbors a total of 112 genes including 71 protein-coding genes (PCGs), 26 transfer RNA genes (tRNAs), three ribosomal RNA genes (rRNAs), three free-standing open reading frames (s) and nine intronic s, and ten introns in seven genes (, , , , , , and ). The maximum likelihood (ML) phylogenomic analysis shows that firstly groups with , and then these two species together with the -- subclade form a monophyletic clade, lineage II. chloroplast genome is the only one in lineage II where the reversal of a collinear block of two genes (-) did not occur, and its genome structure is consistent with that of most chloroplast genomes in lineage I, indicating that the similarity of genome structure is not completely related to the genetic relationship of species. Our genomic data will facilitate the development of specific high-resolution chloroplast molecular markers for rapid identification of , and help us understand its population diversity and genetic characteristics on a global scale.
林奈于1753年（绿藻纲，绿藻门）记载的[物种名称未给出]是一种海洋大型绿藻，分布于中国黄海和渤海沿岸。在此，构建了[物种名称未给出]的完整叶绿体基因组并进行了比较分析。[物种名称未给出]的叶绿体基因组是一个99,041碱基对的环状分子，共包含112个基因，其中包括71个蛋白质编码基因（PCGs）、26个转运RNA基因（tRNAs）、3个核糖体RNA基因（rRNAs）、3个独立的开放阅读框（s）和9个内含子s，以及7个基因（[基因名称未给出]、[基因名称未给出]、[基因名称未给出]、[基因名称未给出]、[基因名称未给出]、[基因名称未给出]和[基因名称未给出]）中的10个内含子。最大似然（ML）系统发育基因组分析表明，[物种名称未给出]首先与[另一物种名称未给出]聚类，然后这两个物种与[某一支系名称未给出] - [某一支系名称未给出]亚分支一起形成一个单系分支，即[物种名称未给出]谱系II。[物种名称未给出]的叶绿体基因组是[物种名称未给出]谱系II中唯一两个基因（[基因名称未给出] - [基因名称未给出]）的共线块未发生反转的基因组，其基因组结构与[物种名称未给出]谱系I中的大多数叶绿体基因组一致，表明基因组结构的相似性与[物种名称未给出]物种的亲缘关系并不完全相关。我们的基因组数据将有助于开发用于快速鉴定[物种名称未给出]的特异性高分辨率叶绿体分子标记，并帮助我们在全球范围内了解其种群多样性和遗传特征。
