聚合酶链反应在疑似病毒性前葡萄膜炎单侧病例中的应用
Polymerase chain reaction in unilateral cases of presumed viral anterior uveitis.
作者信息
Shoughy Samir S, Alkatan Hind M, Al-Abdullah Abdulelah A, El-Khani Albarah, de Groot-Mijnes Jolanda Df, Tabbara Khalid F
机构信息
Department of Ophthalmology, The Eye Center and The Eye Foundation for Research in Ophthalmology, Riyadh, Saudi Arabia.
Department of Pathology & Laboratory Medicine and Uveitis Division, King Khaled Eye Specialist Hospital, Riyadh, Saudi Arabia ; Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
出版信息
Clin Ophthalmol. 2015 Dec 14;9:2325-8. doi: 10.2147/OPTH.S93655. eCollection 2015.
BACKGROUND AND OBJECTIVES
Anterior uveitis is the most common form of intraocular inflammation. The main aim of this study was to determine the viral etiology in patients with unilateral cases of anterior uveitis.
PATIENTS AND METHODS
A total of 12 consecutive patients with the diagnosis of idiopathic unilateral anterior uveitis were included prospectively. Aqueous specimens were obtained from each patient by anterior chamber paracentesis and subjected to the detection of viral DNA/RNA genome by polymerase chain reaction assay for herpes simplex virus, varicella zoster virus, cytomegalovirus, Epstein-Barr virus, and rubella virus.
RESULTS
There were six male and six female patients. The mean age was 43 years, with an age range of 11-82 years. All 12 cases presented with unilateral anterior uveitis. In four (33%) patients, polymerase chain reaction was positive for viral genome. Two patients were positive for herpes simplex virus type 1, one patient was positive for cytomegalovirus and one for Epstein-Barr virus.
CONCLUSION
Recent molecular diagnostic assays would help in the identification of the causative agent in patients with unilateral anterior uveitis.
背景与目的
前葡萄膜炎是眼内炎症最常见的形式。本研究的主要目的是确定单侧前葡萄膜炎患者的病毒病因。
患者与方法
前瞻性纳入了12例连续诊断为特发性单侧前葡萄膜炎的患者。通过前房穿刺从每位患者获取房水标本，并采用聚合酶链反应检测单纯疱疹病毒、水痘带状疱疹病毒、巨细胞病毒、爱泼斯坦-巴尔病毒和风疹病毒的病毒DNA/RNA基因组。
结果
男性和女性患者各6例。平均年龄为43岁，年龄范围为11至82岁。所有12例均表现为单侧前葡萄膜炎。4例（33%）患者的聚合酶链反应检测病毒基因组呈阳性。2例患者单纯疱疹病毒1型呈阳性，1例患者巨细胞病毒呈阳性，1例患者爱泼斯坦-巴尔病毒呈阳性。
结论
近期的分子诊断检测有助于识别单侧前葡萄膜炎患者的病原体。
Zotero 插件