Yap Wai-Sum, Gan Chew-Yan, Sim Kae-Shin, Lim Siew-Huah, Low Yun-Yee, Kam Toh-Seok
University Malaysia of Computer Science and Engineering , Jalan Alamanda 2, Precint 1, 62000 Putrajaya, Malaysia.
J Nat Prod. 2016 Jan 22;79(1):230-9. doi: 10.1021/acs.jnatprod.5b00992. Epub 2015 Dec 30.
Eleven new indole alkaloids (1-11) comprising seven aspidofractinine and four eburnane alkaloids, were isolated from the stem-bark extract of Kopsia pauciflora occurring in Malaysian Borneo. The aspidofractinine alkaloids include a ring-contracted, an additional ring-fused, a paucidactine regioisomer, two paucidactine, and one kopsine alkaloid. The structures of several of these alkaloids were also confirmed by X-ray diffraction analyses. The bisindole alkaloids isolated, norpleiomutine and kopsoffinol, showed in vitro growth inhibitory activity against human PC-3, HCT-116, MCF-7, and A549 cells and moderate effects in reversing multidrug-resistance in vincristine-resistant human KB cells.
从马来西亚婆罗洲产的少花蕊木茎皮提取物中分离出11种新的吲哚生物碱（1-11），包括7种阿朴菲宁和4种伊波加因生物碱。阿朴菲宁生物碱包括一种环收缩的、一种额外环稠合的、一种少花碱区域异构体、两种少花碱和一种蕊木碱生物碱。其中几种生物碱的结构也通过X射线衍射分析得到证实。分离得到的双吲哚生物碱，去甲普列奥穆汀和蕊木酚，对人PC-3、HCT-116、MCF-7和A549细胞显示出体外生长抑制活性，并对长春新碱耐药的人KB细胞的多药耐药逆转有中等作用。
