Lin Saichai, Gan Ning, Zhang Jiabin, Qiao Li, Chen Yinji, Cao Yuting
The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China.
The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China.
Talanta. 2016;149:266-274. doi: 10.1016/j.talanta.2015.11.062. Epub 2015 Nov 26.
In this paper, a novel aptamer-functionalized stir bar sorptive extraction (SBSE) was developed for selective enrichment of the low abundance polychlorinated biphenyls (PCBs) from fish samples. This approach was based on the immobilization of aptamer which could recognize 2,3',5,5'-tetrachlorobiphenyl (PCB72) and 2',3',4',5,5'-pentachlorobiphenyl (PCB106) on one kind of metal-organic frameworks (Zn4O(BDC)3, MOF-5). MOF-5 as a substrate was prepared by potential-controlled cathodic electrodeposition on stainless steel. This aptamer-functionalized stir bar sorptive extraction (Apt-MOF SBSE) fiber could be facile synthesized in one-step. PCB72 and PCB106 were employed as target analytes for selective extraction by the developed method. The adsorbed targets could be desorbed easily in pH 3.0 100mM glycine-HCl buffers and then extracted by the methylene chloride. Afterwards, the detection was carried out with gas chromatography-tandem mass spectrometry (GC-MS). The Apt-MOF SBSE pretreatment coupled with GC-MS exhibited high selectivity, good binding capacity, stability and reproducibility for the detection of PCBs. It provided a linear range of 0.02-250ngmL(-1) with a good coefficient of determination (R(2)=0.9991-0.9996) and the detection limit was 0.003-0.004ngmL(-1). More importantly, the method was successfully utilized for the determination of PCBs in fish samples with good enrichment factor (1930-2304). Therefore, this new SBSE coating opens up the possibility of selective enrichment of a given target PCBs from complex fish samples.
本文开发了一种新型适体功能化搅拌棒吸附萃取（SBSE）方法，用于从鱼样中选择性富集低丰度多氯联苯（PCBs）。该方法基于将能识别2,3',5,5'-四氯联苯（PCB72）和2',3',4',5,5'-五氯联苯（PCB106）的适体固定在一种金属有机框架（Zn4O(BDC)3，MOF-5）上。通过在不锈钢上进行电位控制阴极电沉积制备了作为基底的MOF-5。这种适体功能化搅拌棒吸附萃取（Apt-MOF SBSE）纤维可一步简便合成。采用PCB72和PCB106作为目标分析物，通过所开发的方法进行选择性萃取。吸附的目标物可在pH 3.0的100mM甘氨酸 - 盐酸缓冲液中轻松解吸，然后用二氯甲烷萃取。之后，采用气相色谱 - 串联质谱（GC-MS）进行检测。Apt-MOF SBSE预处理结合GC-MS在检测PCBs时表现出高选择性、良好的结合能力、稳定性和重现性。其线性范围为0.02 - 250ngmL(-1)，决定系数良好（R(2)=0.9991 - 0.9996），检测限为0.003 - 0.004ngmL(-1)。更重要的是，该方法成功用于测定鱼样中的PCBs，富集因子良好（1930 - 2304）。因此，这种新型SBSE涂层为从复杂鱼样中选择性富集特定目标PCBs开辟了可能性。
