Lin Saichai, Gan Ning, Cao Yuting, Chen Yinji, Jiang Qianli
The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China.
The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China.
J Chromatogr A. 2016 May 13;1446:34-40. doi: 10.1016/j.chroma.2016.04.016. Epub 2016 Apr 7.
In this paper, a novel dispersive solid phase extraction (dSPE) adsorbent based on aptamer-functionalized magnetic metal-organic framework material was developed for selective enrichment of the trace polychlorinated biphenyls (PCBs) from soil sample. Firstly, we developed a simple, versatile synthetic strategy to prepare highly reproducible magnetic amino-functionalized UiO-66 (Fe3O4@PDA@UiO-66-NH2) by using polydopamine (PDA) as covalent linker. Then amino-functionalized aptamers which can recognize 2,3',5,5'-tetrachlorobiphenyl (PCB72), 2',3',4',5,5'-pentachlorobiphenyl (PCB106) were covalent immobilized on UiO-66-NH2 through coupling reagent of glutaraldehyde. Aptamer-functionalized adsorbent (Fe3O4@PDA@UiO-66-Apt) can specifically capture PCBs from complex matrix with high adsorption capacity based on the specific affinity of aptamer towards target. Moreover, the adsorbent can be easily isolated from the solution through magnetic separation after extraction. Afterwards, the detection was carried out with gas chromatography tandem mass spectrometry (GC-MS). The selective dSPE pretreatment coupled with GC-MS possessed high selectivity, good binding capacity, stability, repeatability and reproducibility for the extraction of PCBs. Furthermore, the adsorbent possessed good mechanical stability which can be applied in replicate at least for 60 extraction cycles with recovery over 80%. It provided a linear range of 0.02-400ngmL(-1) with a good correlation coefficient (R(2)=0.9994-0.9996), and the limit of detection was found to be 0.010-0.015ngmL(-1). The method was successfully utilized for the determination of PCBs in soil samples.
本文开发了一种基于适体功能化磁性金属有机框架材料的新型分散固相萃取（dSPE）吸附剂，用于从土壤样品中选择性富集痕量多氯联苯（PCBs）。首先，我们开发了一种简单、通用的合成策略，以聚多巴胺（PDA）为共价连接剂，制备具有高重现性的磁性氨基功能化UiO-66（Fe3O4@PDA@UiO-66-NH2）。然后，通过戊二醛偶联剂将能够识别2,3',5,5'-四氯联苯（PCB72）、2',3',4',5,5'-五氯联苯（PCB106）的氨基功能化适体共价固定在UiO-66-NH2上。基于适体对目标物的特异性亲和力，适体功能化吸附剂（Fe3O4@PDA@UiO-66-Apt）能够从复杂基质中特异性捕获PCBs，具有高吸附容量。此外，萃取后通过磁分离可轻松从溶液中分离出吸附剂。之后，采用气相色谱串联质谱（GC-MS）进行检测。选择性dSPE预处理结合GC-MS对PCBs的萃取具有高选择性、良好的结合能力、稳定性、重复性和重现性。此外，该吸附剂具有良好的机械稳定性，至少可重复应用60次萃取循环，回收率超过80%。其线性范围为0.02 - 400ngmL(-1)，相关系数良好（R(2)=0.9994 - 0.999），检测限为0.010 - 0.015ngmL(-1)。该方法成功用于土壤样品中PCBs的测定。
