Wahyuni Wulan T, Ivandini Tribidasari A, Saepudin Endang, Einaga Yasuaki
Department of Chemistry, Faculty of Mathematics and Sciences, University of Indonesia, Kampus UI Depok, Jakarta 16424, Indonesia.
Department of Chemistry, Faculty of Mathematics and Sciences, University of Indonesia, Kampus UI Depok, Jakarta 16424, Indonesia.
Anal Biochem. 2016 Mar 15;497:68-75. doi: 10.1016/j.ab.2015.12.003. Epub 2015 Dec 21.
Gold nanoparticles-modified boron-doped diamond (AuNPs-BDD) electrodes, which were prepared with a self-assembly deposition of AuNPs at amine-terminated boron-doped diamond, were examined for voltammetric detection of neuraminidase (NA). The detection method was performed based on the difference of electrochemical responses of zanamivir at gold surface before and after the reaction with NA in phosphate buffer solution (PBS, pH 5.5). A linear calibration curve for zanamivir in 0.1 M PBS in the absence of NA was achieved in the concentration range of 1 × 10(-6) to 1 × 10(-5) M (R(2) = 0.99) with an estimated limit of detection (LOD) of 2.29 × 10(-6) M. Furthermore, using its reaction with 1.00 × 10(-5) M zanamivir, a linear calibration curve of NA can be obtained in the concentration range of 0-12 mU (R(2) = 0.99) with an estimated LOD of 0.12 mU. High reproducibility was shown with a relative standard deviation (RSD) of 1.14% (n = 30). These performances could be maintained when the detection was performed in mucin matrix. Comparison performed using gold-modified BDD (Au-BDD) electrodes suggested that the good performance of the detection method is due to the stability of the gold particles position at the BDD surface.
通过在胺基封端的硼掺杂金刚石上自组装沉积金纳米颗粒制备的金纳米颗粒修饰硼掺杂金刚石（AuNPs-BDD）电极，用于神经氨酸酶（NA）的伏安检测。该检测方法基于扎那米韦在磷酸盐缓冲溶液（PBS，pH 5.5）中与NA反应前后在金表面的电化学响应差异。在不存在NA的情况下，在0.1 M PBS中获得了扎那米韦的线性校准曲线，浓度范围为1×10⁻⁶至1×10⁻⁵ M（R² = 0.99），估计检测限（LOD）为2.29×10⁻⁶ M。此外，利用其与1.00×10⁻⁵ M扎那米韦的反应，可在0 - 12 mU的浓度范围内获得NA的线性校准曲线（R² = 0.99），估计LOD为0.12 mU。相对标准偏差（RSD）为1.14%（n = 30），显示出高重现性。当在粘蛋白基质中进行检测时，这些性能可以保持。使用金修饰的BDD（Au-BDD）电极进行的比较表明，该检测方法的良好性能归因于金颗粒在BDD表面位置的稳定性。
