Lucena Filho José Hardman Sátiro de, Lima Rennaly de Freitas, Medeiros Ana Claudia Dantas de, Pereira Jozinete Vieira, Granville-Garcia Ana Flávia, Costa Edja Maria Melo de Brito
Department of Dentistry, Paraiba State University, Campina Grande, Paraiba, Brazil.
Department of Pharmacy, Paraiba State University, Campina Grande, Paraiba, Brazil.
J Contemp Dent Pract. 2015 Nov 1;16(11):854-8. doi: 10.5005/jp-journals-10024-1770.
The aim of the present study was to evaluate the antibacterial and antifungal potential in vitro of Momordica charantia L. against the microorganisms of clinical interest (standard strains and multiresistant isolates) in order to aggregate scientific information in relation to its use as a therapeutic product.
M. charantia L. plant material was acquired in municipality of Malta, Paraiba, Brazil. The extract was obtained through maceration, filtration and then concentrated under reduced pressure in a rotary evaporator, resulting in a dough, and was then dried in an oven for 72 hours at 40°C. Antimicrobial action of ethanolic extract of seed M. charantia L. was evaluated based on the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) against standard strains of bacteria, isolates multiresistant bacteria and Candida species, by microdilution in broth method.
All organisms were sensitive to the extract, being considered strong antimicrobial activity (MIC and MBC/MFC < 0.125 mg/ml).
The M. charantia L. showed strong antimicrobial potential, with bactericidal and fungicidal profile, there is the prospect to constitute a new therapeutic strategy for the control of infections, particularly in multiresistant strains.
The use of medicinal plants in treatment of infectious processes have an important function nowadays, due to the limitations of the use of synthetic antibiotics available, related specifically to the microbial resistance emergence.
本研究旨在评估苦瓜对具有临床意义的微生物（标准菌株和多重耐药菌株）的体外抗菌和抗真菌潜力，以便收集有关其作为治疗产品用途的科学信息。
苦瓜植物材料采自巴西帕拉伊巴州马耳他市。通过浸渍、过滤，然后在旋转蒸发仪中减压浓缩得到提取物，得到一种糊状物，然后在40℃烘箱中干燥72小时。采用肉汤微量稀释法，基于对细菌标准菌株、多重耐药细菌分离株和念珠菌属的最低抑菌浓度（MIC）、最低杀菌浓度（MBC）和最低杀真菌浓度（MFC），评估苦瓜种子乙醇提取物的抗菌作用。
所有受试微生物对该提取物均敏感，显示出较强的抗菌活性（MIC及MBC/MFC＜0.125mg/ml）。
苦瓜显示出较强的抗菌潜力，具有杀菌和杀真菌作用，有望构成一种控制感染的新治疗策略，尤其是针对多重耐药菌株。
由于现有合成抗生素使用的局限性，特别是与微生物耐药性出现相关的问题，药用植物在治疗感染过程中的应用目前具有重要作用。
