Zorina Anna, Sinetova Maria A, Kupriyanova Elena V, Mironov Kirill S, Molkova Irina, Nazarenko Lyudmila V, Zinchenko Vladislav V, Los Dmitry A
Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow, Russia, 127276.
Moscow City Teacher Training University, 2nd Agricultural Passage 4, Moscow, Russia, 129226.
Photosynth Res. 2016 Dec;130(1-3):11-17. doi: 10.1007/s11120-015-0214-x. Epub 2015 Dec 31.
High affinity transport of manganese ions (Mn) in cyanobacteria is carried by an ABC-type transporter, encoded by the mntCAB operon, which is derepressed by the deficiency of Mn. Transcription of this operon is negatively regulated by the two-component system consisting of a sensory histidine kinase ManS and DNA-binding response regulator ManR. In this study, we examined two Synechocystis mutants, defective in ManS and ManR. These mutants were unable to grow on high concentrations of manganese. Furthermore, they were sensitive to high light intensity and unable to recover after short-term photoinhibition. Under standard illumination and Mn concentration, mutant cells revealed the elevated levels of transcripts of genes involved in the formation of Photosystem II (psbA, psbD, psbC, pap-operon). This finding suggests that, in mutant cells, the PSII is sensitive to high concentrations of Mn even at relatively low light intensity.
蓝藻中锰离子（Mn）的高亲和力转运由mntCAB操纵子编码的ABC型转运蛋白负责，该操纵子在锰缺乏时被去阻遏。该操纵子的转录受由传感组氨酸激酶ManS和DNA结合反应调节因子ManR组成的双组分系统负调控。在本研究中，我们检测了两个在ManS和ManR方面有缺陷的集胞藻突变体。这些突变体无法在高浓度锰上生长。此外，它们对高光强度敏感，并且在短期光抑制后无法恢复。在标准光照和锰浓度下，突变体细胞中参与光系统II形成的基因（psbA、psbD、psbC、pap操纵子）的转录水平升高。这一发现表明，在突变体细胞中，即使在相对低的光照强度下，光系统II对高浓度的锰也敏感。
