Ghosh Sougata, Nitnavare Rahul, Dewle Ankush, Tomar Geetanjali B, Chippalkatti Rohan, More Piyush, Kitture Rohini, Kale Sangeeta, Bellare Jayesh, Chopade Balu A
Institute of Bioinformatics and Biotechnology, University of Pune, Pune, India.
Department of Applied Physics, Defense Institute of Advanced Technology, Pune, India.
Int J Nanomedicine. 2015 Dec 17;10:7477-90. doi: 10.2147/IJN.S91579. eCollection 2015.
Medicinal plants serve as rich sources of diverse bioactive phytochemicals that might even take part in bioreduction and stabilization of phytogenic nanoparticles with immense therapeutic properties. Herein, we report for the first time the rapid efficient synthesis of novel platinum-palladium bimetallic nanoparticles (Pt-PdNPs) along with individual platinum (PtNPs) and palladium (PdNPs) nanoparticles using a medicinal plant, Dioscorea bulbifera tuber extract (DBTE). High-resolution transmission electron microscopy revealed monodispersed PtNPs of size 2-5 nm, while PdNPs and Pt-PdNPs between 10 and 25 nm. Energy dispersive spectroscopy analysis confirmed 30.88% ± 1.73% elemental Pt and 68.96% ± 1.48% elemental Pd in the bimetallic nanoparticles. Fourier transform infrared spectra indicated strong peaks at 3,373 cm(-1), attributed to hydroxyl group of polyphenolic compounds in DBTE that might play a key role in bioreduction in addition to the sharp peaks at 2,937, 1,647, 1,518, and 1,024 cm(-1), associated with C-H stretching, N-H bending in primary amines, N-O stretching in nitro group, and C-C stretch, respectively. Anticancer activity against HeLa cells showed that Pt-PdNPs exhibited more pronounced cell death of 74.25% compared to individual PtNPs (12.6%) or PdNPs (33.15%). Further, Pt-PdNPs showed an enhanced scavenging activity against 2,2-diphenyl-1-picrylhydrazyl, superoxide, nitric oxide, and hydroxyl radicals.
药用植物是多种具有生物活性的植物化学物质的丰富来源，这些植物化学物质甚至可能参与具有巨大治疗特性的植物源纳米颗粒的生物还原和稳定过程。在此，我们首次报告了使用药用植物黄独块茎提取物（DBTE）快速高效地合成新型铂钯双金属纳米颗粒（Pt-PdNPs）以及单独的铂（PtNPs）和钯（PdNPs）纳米颗粒。高分辨率透射电子显微镜显示，PtNPs的尺寸为2-5nm，呈单分散状态，而PdNPs和Pt-PdNPs的尺寸在10至25nm之间。能量色散光谱分析证实，双金属纳米颗粒中元素Pt的含量为30.88%±1.73%，元素Pd的含量为68.96%±1.48%。傅里叶变换红外光谱表明，在3373cm-1处有强峰，这归因于DBTE中多酚类化合物的羟基，除了在2937、1647、1518和1024cm-1处的尖峰外，该羟基可能在生物还原中起关键作用，这些尖峰分别与C-H伸缩、伯胺中的N-H弯曲、硝基中的N-O伸缩和C-C伸缩有关。对HeLa细胞的抗癌活性表明，与单独的PtNPs（12.6%）或PdNPs（33.15%）相比，Pt-PdNPs表现出更明显的细胞死亡，死亡率为74.25%。此外，Pt-PdNPs对2,2-二苯基-1-苦基肼、超氧化物、一氧化氮和羟基自由基的清除活性增强。
