Department of Biochemistry and Microbiology, University of Chemistry and Technology, Technicka 5, 166 28 Prague 6, Czech Republic.
Department of Solid State Engineering, University of Chemistry and Technology, Technicka 5, 166 28 Prague 6, Czech Republic.
Int J Mol Sci. 2021 Jul 23;22(15):7892. doi: 10.3390/ijms22157892.
Although some metallic nanoparticles (NPs) are commonly used in the food processing plants as nanomaterials for food packaging, or as coatings on the food handling equipment, little is known about antimicrobial properties of palladium (PdNPs) and platinum (PtNPs) nanoparticles and their potential use in the food industry. In this study, common food-borne pathogens Infantis, , and were tested. Both NPs reduced viable cells with the log CFU reduction of 0.3-2.4 (PdNPs) and 0.8-2.0 (PtNPs), average inhibitory rates of 55.2-99% for PdNPs and of 83.8-99% for PtNPs. However, both NPs seemed to be less effective for biofilm formation and its reduction. The most effective concentrations were evaluated to be 22.25-44.5 mg/L for PdNPs and 50.5-101 mg/L for PtNPs. Furthermore, the interactions of tested NPs with bacterial cell were visualized by transmission electron microscopy (TEM). TEM visualization confirmed that NPs entered bacteria and caused direct damage of the cell walls, which resulted in bacterial disruption. The in vitro cytotoxicity of individual NPs was determined in primary human renal tubular epithelial cells (HRTECs), human keratinocytes (HaCat), human dermal fibroblasts (HDFs), human epithelial kidney cells (HEK 293), and primary human coronary artery endothelial cells (HCAECs). Due to their antimicrobial properties on bacterial cells and no acute cytotoxicity, both types of NPs could potentially fight food-borne pathogens.
虽然一些金属纳米粒子（NPs）通常被用作食品包装的纳米材料，或作为食品处理设备的涂层，用于食品加工厂，但对于钯（PdNPs）和铂（PtNPs）纳米粒子的抗菌特性及其在食品工业中的潜在用途知之甚少。在这项研究中，测试了常见的食源性病原体 婴儿、、和。两种 NPs 都减少了活菌，其活菌 CFU 减少了 0.3-2.4（PdNPs）和 0.8-2.0（PtNPs），对 PdNPs 的平均抑制率为 55.2-99%，对 PtNPs 的平均抑制率为 83.8-99%。然而，两种 NPs 似乎对生物膜形成及其减少的效果较小。评估最有效浓度分别为 22.25-44.5 mg/L 的 PdNPs 和 50.5-101 mg/L 的 PtNPs。此外，通过透射电子显微镜（TEM）观察测试 NPs 与细菌细胞的相互作用。TEM 可视化证实 NPs 进入细菌并直接破坏细胞壁，导致细菌破裂。通过原代人肾小管上皮细胞（HRTECs）、人角质形成细胞（HaCat）、人真皮成纤维细胞（HDFs）、人肾上皮细胞（HEK 293）和原代人冠状动脉内皮细胞（HCAECs）确定了单个 NPs 的体外细胞毒性。由于它们对细菌细胞的抗菌特性和无急性细胞毒性，这两种类型的 NPs 都有可能对抗食源性病原体。
