Lin Qiuyun, Wang Wenyan, Ren Yakun, Jiang Yimei, Sun Ailing, Qian Ying, Zhang Yifei, He Niqing, Hang Ngo Thi, Liu Zhou, Li Linfang, Liu Linglong, Jiang Ling, Wan Jianmin
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University , Nanjing 210095 , China.
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University , Nanjing 210095 , China ; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences , Beijing 100081 , China.
Breed Sci. 2015 Dec;65(5):411-9. doi: 10.1270/jsbbs.65.411. Epub 2015 Dec 1.
Seed storability in rice (Oryza sativa L.) is an important agronomic trait. Two segregating populations with N22 (indica) as a common parent, viz. a set of 122 backcross-inbred lines (BILs) derived from the backcross Nanjing35 (japonica)/N22//Nanjing35 and another population comprising 189 recombinant inbred lines (RILs) from the cross of USSR5 (japonica) and N22, were studied to detect quantitative trait loci (QTL) controlling seed storability. Germination percentage (GP) was used to evaluate seed storability after aging treated under three different conditions, viz. natural, artificial and combined aging treatments. A total of seven QTLs were identified on chromosomes 1, 2, 5, 6 and 9. Among them, a major QTL, qSSn-9, was common in the two populations. In contrast, four QTLs (qSSnj-2-1, qSSn-2-2, qSSn-5 and qSSn-6) were detected in BILs and the QTL qSSn-1 was identified in RILs, which was a new QTL for seed storability. The N22-derived alleles increased the seed storability at all the loci except qSSnj-2-1. We also investigated the effect of QTLs using five selected lines with high storability from BILs and verified qSSn-5 with a near-isogenic line (NIL). These results provide an opportunity for pyramiding or map-based cloning major QTLs for seed storability in rice.
水稻（Oryza sativa L.）种子的耐贮藏性是一项重要的农艺性状。以N22（籼稻）为共同亲本构建了两个分离群体，即一组122个回交自交系（BILs），来源于南京35（粳稻）/N22//南京35的回交后代，以及另一个群体，由189个苏联5号（粳稻）与N22杂交的重组自交系（RILs）组成，用于检测控制种子耐贮藏性的数量性状位点（QTL）。在自然、人工和复合老化三种不同条件下对种子进行老化处理后，用发芽率（GP）来评估种子的耐贮藏性。在第1、2、5、6和9号染色体上共鉴定出7个QTL。其中，一个主要QTL，qSSn - 9，在两个群体中都存在。相比之下，在BILs中检测到4个QTL（qSSnj - 2 - 1、qSSn - 2 - 2、qSSn - 5和qSSn - 6），在RILs中鉴定出QTL qSSn - 1，这是一个新的种子耐贮藏性QTL。除qSSnj - 2 - 1外，所有位点上来源于N22的等位基因都提高了种子的耐贮藏性。我们还使用从BILs中选出的5个耐贮藏性高的品系研究了QTL的效应，并用近等基因系（NIL）验证了qSSn - 5。这些结果为聚合或基于图谱克隆水稻种子耐贮藏性的主要QTL提供了机会。
