Wu Fangxi, Wei Yidong, Zhu Yongsheng, Luo Xi, He Wei, Wang Yingheng, Cai Qiuhua, Xie Huaan, Xie Guosheng, Zhang Jianfu
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350019, China.
Ministry of Agriculture and Rural Affairs of the People's Republic of China, Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Metabolites. 2025 Jan 5;15(1):19. doi: 10.3390/metabo15010019.
Seed storability is a crucial agronomic trait and indispensable for the safe storage of rice seeds and grains. Nevertheless, the metabolite mechanisms governing rice seed storability under natural conditions are still poorly understood.
Therefore, the seed storage tolerance of global rice core germplasms stored for two years under natural aging conditions were identified, and two extreme groups with different seed storabilities from the rice group were analyzed using the UPLC-MS/MS metabolomic strategy.
Our results proved that the different rice core accessions showed significant variability in storage tolerance, and the metabolite analysis of the two rice pools exhibited different levels of storability. A total of 103 differentially accumulated metabolites (DAMs) between the two pools were obtained, of which 38 were up-regulated and 65 were down-regulated, respectively. Further analysis disclosed that the aging-resistant rice accessions had higher accumulation levels of flavonoids, terpenoids, phenolic acids, organic acids, lignans, and coumarins while exhibiting lower levels of lipids and alkaloids compared to the storage-sensitive rice accessions. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated that several biosynthesis pathways were involved in the observed metabolite differences, including alpha-linolenic acid metabolism, butanoate metabolism, and propanoate metabolism. Notably, inhibition of the linolenic acid metabolic pathway could enhance seed storability. Additionally, increased accumulations of organic acids, such as succinic acid, D-malic acid, and methylmalonic acid, in the butanoate and propanoate metabolisms were identified as a beneficial factor for seed storage.
These new findings will deepen our understanding of the underlying mechanisms governing rice storability.
种子耐贮性是一项关键的农艺性状，对水稻种子和谷物的安全储存至关重要。然而，在自然条件下控制水稻种子耐贮性的代谢物机制仍知之甚少。
因此，鉴定了在自然老化条件下储存两年的全球水稻核心种质的种子贮藏耐受性，并使用超高效液相色谱-串联质谱代谢组学策略分析了来自水稻组的具有不同种子耐贮性的两个极端群体。
我们的结果证明，不同的水稻核心种质在贮藏耐受性方面表现出显著差异，并且对两个水稻群体的代谢物分析显示出不同水平的耐贮性。在两个群体之间共获得了103种差异积累代谢物（DAMs），其中38种上调，65种下调。进一步分析表明，与贮藏敏感的水稻种质相比，抗老化水稻种质中黄酮类、萜类、酚酸、有机酸、木脂素和香豆素的积累水平较高，而脂质和生物碱的水平较低。京都基因与基因组百科全书（KEGG）通路富集分析表明，几种生物合成通路参与了观察到的代谢物差异，包括α-亚麻酸代谢、丁酸代谢和丙酸代谢。值得注意的是，抑制亚麻酸代谢途径可以提高种子耐贮性。此外，在丁酸和丙酸代谢中琥珀酸、D-苹果酸和甲基丙二酸等有机酸积累的增加被确定为种子贮藏的有利因素。
这些新发现将加深我们对控制水稻耐贮性潜在机制的理解。
