微小RNA多态性与甲状腺乳头状癌易感性之间的关联。
Association between microRNA polymorphisms and papillary thyroid cancer susceptibility.
作者信息
Dong Gang, Zhang Ruifang, Xu Jingjing, Guo Yanfei
机构信息
Department of Ultrasound, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
出版信息
Int J Clin Exp Pathol. 2015 Oct 1;8(10):13450-7. eCollection 2015.
OBJECTIVES
Papillary thyroid cancer (PTC) is the most common subtype of thyroid cancer, which accounts for 80-90% of all thyroid cancer cases. Though the pathological mechanism hasn't been fully understood, it is reported that both environmental and genetic factor may contribute to the PTC susceptibility. MicroRNAs (miRNAs) are small non-coding RNA molecules which function as the suppressors to participate in a variety of biological processes. Accumulating evidence suggests that polymorphisms of miRNAs were associated with the tumorigenesis of various cancers, including PTC. In this article, we focus on the association between four common microRNA polymorphisms (miR-146a, miR-608, miR-933, and miR-149) and PTC risk in a Han Chinese population.
METHODS
In this case-control study, we recruited 1,398 participants in total, including 369 PTC patients, 278 patients with thyroid benign nodules (BN) and 751 normal controls. The miRNAs polymorphisms were genotyped and analyzed by using MALDI-TOF mass spectrometry. The odd ratios and their 95% confidence interval (95% CI) were calculated to evaluate the association between miRNAs polymorphisms and PTC risk. Furthermore, a meta-analysis based on previous studies was conducted to comprehensively assess the diagnostic performance of miR-146a in the PTC diagnosis.
RESULTS
The miR-146a polymorphisms were shown to be significantly correlated with elevated risk of PTC under the heterozygous, homozygous, dominant and allelic models by comparing the genotype distribution between PTC cases and healthy controls, as well as between PTC cases and BN cases. However, the result of meta-analysis showed no significant association between miR-146a polymorphisms and PTC risk.
CONCLUSIONS
Our study indicated that the miR-146a polymorphism was significantly associated with PTC risk. In contrast, meta-analysis revealed no evidence of association between miR-146a variants and PTC risk. Further studies are required to elucidate the role of miR-146a in the etiology of PTC.
目的
甲状腺乳头状癌（PTC）是甲状腺癌最常见的亚型，占所有甲状腺癌病例的80 - 90%。尽管其病理机制尚未完全明确，但据报道环境和遗传因素都可能导致PTC易感性。微小RNA（miRNA）是小的非编码RNA分子，作为抑制剂参与多种生物学过程。越来越多的证据表明，miRNA的多态性与包括PTC在内的各种癌症的肿瘤发生有关。在本文中，我们聚焦于四种常见的微小RNA多态性（miR - 146a、miR - 608、miR - 933和miR - 149）与中国汉族人群PTC风险之间的关联。
方法
在这项病例对照研究中，我们共招募了1398名参与者，包括369例PTC患者、278例甲状腺良性结节（BN）患者和751名正常对照。采用基质辅助激光解吸电离飞行时间质谱法对miRNA多态性进行基因分型和分析。计算比值比及其95%置信区间（95%CI）以评估miRNA多态性与PTC风险之间的关联。此外，基于先前的研究进行了荟萃分析，以全面评估miR - 146a在PTC诊断中的诊断性能。
结果
通过比较PTC病例与健康对照以及PTC病例与BN病例之间的基因型分布，发现在杂合子、纯合子、显性和等位基因模型下，miR - 146a多态性与PTC风险升高显著相关。然而，荟萃分析结果显示miR - 146a多态性与PTC风险之间无显著关联。
结论
我们的研究表明，miR - 146a多态性与PTC风险显著相关。相比之下，荟萃分析未发现miR - 146a变异与PTC风险之间存在关联的证据。需要进一步研究以阐明miR - 146a在PTC病因学中的作用。
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