Department of Head & Neck Surgery, Cancer Hospital, Fudan University, Shanghai, China.
PLoS One. 2013;8(2):e56638. doi: 10.1371/journal.pone.0056638. Epub 2013 Feb 22.
Rs2910164, a Single nucleotide polymorphism (SNP) located in the precursor microRNA sequence of miR-146a, is the only MicroRNA sequence SNP studied in papillary thyroid cancer (PTC). Association studies had been performed in US and UK-Northern European populations, but results were inconsistence. This study evaluated the association between rs2910164 and the risk of PTC as well as benign thyroid tumor (BN), and examined the clinicopathological characteristics of PTC and BN for different genotypes.
This case-control study genotyped rs2910164 in 753 PTCs, 484 BNs and 760 controls in a Chinese Han population. Clinicopathological and genetic data were collected and compared. Multivariate logistic regression was performed to calculate adjusted odds ratios (ORs).
There were no differences in rs2910164 genotype distributions between the three groups. PTC cases with three genotypes (CC, CG, GG) had similar clinicopathological characteristics except the existence of "para-cancer" BN (PTC/BN, P = 0.006). PTC/BN patients were older (P = 0.009), and had smaller cancer lesions (P<0.001), lower serum thyrotropin levels (1.82±1.42 vs. 2.21±1.74, P = 0.04), and lower rates of level VI lymph node metastasis (20.8% vs. 52.7%, P<0.001) and lateral neck lymph node metastasis (11.5% vs. 23.0%, P = 0.011) compared with PTC only. Then we supposed a possible progression from BN to PTC which may involve rs2910164 in and performed a multivariate logistic regression analysis of PTC/BN and BN cases to determine risk factors of this progression. Results showed that the rs2910164 GG homozygote (OR = 2.25, 95% CI 1.22-4.14, P = 0.01) was the only risk factor in this study.
Rs2910164 was not associated with increased risk of PTC and BN in Chinese patients, but may play a latent role in the transformation from BN to PTC.
位于 miR-146a 前体 microRNA 序列中的单核苷酸多态性（SNP）rs2910164 是研究甲状腺乳头状癌（PTC）的唯一 microRNA 序列 SNP。在美国和英国-北欧人群中进行了关联研究，但结果不一致。本研究评估了 rs2910164 与 PTC 和良性甲状腺肿瘤（BN）风险之间的关联，并检查了不同基因型 PTC 和 BN 的临床病理特征。
本病例对照研究在中国汉族人群中对 753 例 PTC、484 例 BN 和 760 例对照进行了 rs2910164 基因分型。收集并比较临床病理和遗传数据。采用多变量 logistic 回归计算调整后的优势比（OR）。
三组间 rs2910164 基因型分布无差异。三种基因型（CC、CG、GG）的 PTC 病例具有相似的临床病理特征，除存在“癌旁”BN（PTC/BN，P=0.006）外。PTC/BN 患者年龄较大（P=0.009），肿瘤病变较小（P<0.001），血清促甲状腺激素水平较低（1.82±1.42 vs. 2.21±1.74，P=0.04），水平 VI 淋巴结转移率较低（20.8% vs. 52.7%，P<0.001）和侧颈淋巴结转移率较低（11.5% vs. 23.0%，P=0.011）与单纯 PTC 相比。然后，我们推测可能存在从 BN 到 PTC 的进展，这可能涉及 rs2910164，并对 PTC/BN 和 BN 病例进行多变量 logistic 回归分析，以确定该进展的危险因素。结果表明，rs2910164 GG 纯合子（OR=2.25，95%CI 1.22-4.14，P=0.01）是本研究中的唯一危险因素。
rs2910164 与中国患者 PTC 和 BN 风险增加无关，但可能在 BN 向 PTC 转化中发挥潜在作用。
