Department of Pesticide Science, College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, PR China; Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, PR China.
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, PR China.
Ecotoxicol Environ Saf. 2016 Apr;126:78-84. doi: 10.1016/j.ecoenv.2015.12.007. Epub 2015 Dec 23.
Tebuconazole is an effective chiral fungicide, and previous studies have demonstrated that tebuconazole enantiomers exhibit enantioselective toxicity to non-target aquatic organisms. Thus, the aim of the present study was to investigate the chiral bioaccumulation behavior of tebuconazole in zebrafish (Danio rerio). Two exposure concentrations (0.107 and 1.07 mg/L) of tebuconazole were used. The uptake experiments lasted for 8 days, and subsequently, the zebrafish were transferred to another clean tank containing water without tebuconazole for depuration experiments (up to 14 days). A significant trend in enantioselective bioaccumulation was observed in these zebrafish with the preferential accumulation of (-)-R-tebuconazole at two dose levels. The results of the depuration experiments indicated that the degradation of (-)-R-tebuconazole in zebrafish was slower than that of (+)-S-tebuconazole. The BCFk values for (+)-S-tebuconazole and (-)-R-tebuconazole in a low dose of this chemical were 11.22 and 16.25, respectively, while at a high dose, these values were 9.79 and 10.31, respectively. The enantiomer fraction of tebuconazole in zebrafish and water ranged from 0.31-0.49. Hence, future research should focus on the fate of tebuconazole in the aquatic environment at the enantiomer levels.
戊唑醇是一种有效的手性杀菌剂，先前的研究表明，戊唑醇对映体对非靶标水生生物表现出对映选择性毒性。因此，本研究旨在研究戊唑醇在斑马鱼（Danio rerio）中的手性生物累积行为。使用了两个戊唑醇暴露浓度（0.107 和 1.07 mg/L）。摄取实验持续了 8 天，随后将斑马鱼转移到另一个不含戊唑醇的清洁水箱中进行净化实验（长达 14 天）。在这些斑马鱼中观察到了明显的对映体选择性生物累积趋势，两种剂量水平下都优先积累（-）-R-戊唑醇。净化实验的结果表明，（-）-R-戊唑醇在斑马鱼中的降解速度比（+）-S-戊唑醇慢。在这种化学物质的低剂量下，（+）-S-戊唑醇和（-）-R-戊唑醇的 BCFk 值分别为 11.22 和 16.25，而在高剂量下，这些值分别为 9.79 和 10.31。戊唑醇在斑马鱼和水中的对映体分数范围为 0.31-0.49。因此，未来的研究应集中在手性杀菌剂戊唑醇在水环境中的对映体水平上的命运。
